Bioinformatics (2012) 28(20), 2683–2684 The authors of the above article wish to amend the funding acknowledgement, so that it reads as follows: This article was partially supported by the European projects MEPHITIS and GEN2PHEN and by FEDER funds through the Programa Operacional Factores de Competitividade - COMPETE (FCOMP-01-0124-FEDER-014284) and by National Funds through FCT - Foundation for Science and Technology in the scope of the research project ref. PTDC/BIA-GEN/110383/2009. P.G. was supported by FCT-SFRH/BD/71063/2010. The authors apologize for this error.

UNLABELLED: Numerous software applications exist to deal with synthetic gene design, granting the field of heterologous expression a significant support. However, their dispersion requires the access to different tools and online services in order to complete one single project. Analyzing codon usage, calculating codon adaptation index (CAI), aligning orthologs and optimizing genes are just a few examples. A software application, EuGene, was developed for the optimization of multiple gene synthetic design algorithms. In a seamless automatic form, EuGene calculates or retrieves genome data on codon usage (relative synonymous codon usage and CAI), codon context (CPS and codon pair bias), GC content, hidden stop codons, repetitions, deleterious sites, protein primary, secondary and tertiary structures, gene orthologs, species housekeeping genes, performs alignments and identifies genes and genomes. The main function of EuGene is analyzing and redesigning gene sequences using multi-objective optimization techniques that maximize the coding features of the resulting sequence. AVAILABILITY: EuGene is freely available for non-commercial use, at http://bioinformatics.ua.pt/eugene.