Graph-based machine learning methods are valuable tools for identifying and predicting variation in genetic data. In particular, understanding phenotypic effects at the cellular level is an accelerating area in pharmacogenomics. Insight into how drugs or disease affect bio-networks could aid drug development and precision medicine. This article proposes a novel graph-theoretic approach to infer a co-occurrence network from 16S microbiome data, designed specifically for smallsample datasets. Such datasets pose challenges due to sparsity, compositionality, and complex interactions. The methodology includes steps to enrich and statistically filter the inferred networks. The approach extracts informative, feature-rich, biologically meaningful, and statistically significant networks from limited data. While tailored for small datasets, it is broadly applicable and can be extended to multi-omics integration. The method is tested on data from chickens vaccinated and challenged with Eimeria tenella. Genetic reads are processed, and networks inferred to characterize intestinal ecosystems at three disease progression stages. Analysis of network features yields biologically intuitive conclusions using statistical methods. Notably, the distribution of node features evolves with disease progression, and distributions reveal mutualistic and parasitic species clusters. A sub-network consistently appears across all conditions, suggesting a 'persistent microbiome'. A clustering algorithm is also applied to demonstrate the methods utility for downstream analysis.

As the COVID-19 pandemic continues, a growing number of recovered patients report persistent symptoms such as fatigue, muscle weakness, sleep issues, anxiety, and depression, lasting months or even over a year. Severe cases often show significant lung damage and sometimes reduced kidney function. This study examines a dataset from recovered COVID-19 patients, using machine learning to assess the likelihood of developing Post-COVID-19 conditions. We applied several models, including XGBoost, Decision Trees, and Random Forest, to predict outcomes based on data from a specific hospital. Our approach included detailed data preprocessing-filling in missing values, feature engineering, and standardizing data to improve model accuracy and applicability. Results showed the Random Forest model as the most accurate, demonstrating the power of machine learning in making precise predictions from complex health data. Feature importance analysis revealed critical factors predicting Post-COVID-19 conditions, offering vital guidance for healthcare professionals in managing recovered patients.

Abstract The influenza problem has always been an important global issue. It not only affects people’s health problems but is also an essential topic of governments and health care facilities. Early prediction and response is the most effective control method for flu epidemics. It can effectively predict the influenza-like illness morbidity, and provide reliable information to the relevant facilities. For social facilities, it is possible to strengthen epidemic prevention and care for highly sick groups. It can also be used as a reminder for the public. This study collects information on the influenza-like illness emergency department visits to the Taiwan Centers for Disease Control, and the PM2.5 open-source data from the Taiwan Environmental Protection Administration's air quality monitoring network. By using deep learning techniques, the relevance of short-term estimates and the outbreak calculation method can be determined. The techniques are published by the WHO to determine whether the influenza-like illness situation is still in a stage of reasonable control. Finally, historical data and future forecasted data are integrated on the web page for visual presentation, to show the actual regional air quality situation and influenza-like illness data and to predict whether there is an outbreak of influenza in the region.