MOTIVATION: Despite increased availability of genome annotation data, a comprehensive resource for in-depth analysis of splice signal distributions and alternative splicing (AS) patterns in eukaryote genomes is still lacking. To meet this need, we have developed EuSplice--a unique splice-centric database which provides reliable splice signal and AS information for 23 eukaryotes. RESULTS: The EuSplice database contains 95,822 AS events and 2.1 million splice signals associated with over 270,000 protein-coding genes. The intuitive, user-friendly EuSplice web interface has powerful data mining and graphics capabilities for inter-genomic comparative analysis of splice signals, putative cryptic splice sites and AS events. Moreover, the seamless integration of splicing data to extensive gene-specific annotations, such as homolog annotations, functional information, mutations and sequence details makes EuSplice a powerful one-stop information resource for investigating the molecular mechanisms of complex splicing events, disease associations and the evolution of splicing in eukaryotes. AVAILABILITY: http://66.170.16.154/EuSplice. SUPPLEMENTARY INFORMATION: Supplementary tables and figures at Bioinfo online.

Finding genes unequivocally in DNA sequences is one of the key goals of the Human Genome project. The human genome is a 3 billion character long DNA sequence and is estimated to contain about 100000 genes. It has been shown by several biologists that genes in a DNA sequence satisfy certain special properties. We use a combination of these properties to design a serial algorithm for gene-finding. To speed up the process of finding genes in long DNA sequences (of the order of /spl ges/100000 characters), we design a parallel algorithm for gene-finding. We have implemented the parallel gene-finding algorithm on the CM-5 multicomputer as well as on a network of HP Apollo workstations under Parallel Virtual Machine software package. Experimental results indicate that our algorithms predict genes with reasonable accuracy.>