Brain vessel image segmentation can be used as a promising biomarker for better prevention and treatment of different diseases. One successful approach is to consider the segmentation as an image-to-image translation task and perform a conditional Generative Adversarial Network (cGAN) to learn a transformation between two distributions. In this paper, we present a novel multi-view approach, MLP-GAN, which splits a 3D volumetric brain vessel image into three different dimensional 2D images (i.e., sagittal, coronal, axial) and then feed them into three different 2D cGANs. The proposed MLP-GAN not only alleviates the memory issue which exists in the original 3D neural networks but also retains 3D spatial information. Specifically, we utilize U-Net as the backbone for our generator and redesign the pattern of skip connection integrated with the MLP-Mixer [1] which has attracted lots of attention recently. Our model obtains the ability to capture cross-patch information to learn global information with the MLP-Mixer. Extensive experiments are performed on the public brain vessel dataset [2] that show our MLP-GAN outperforms other state-of-the-art methods.

Recently, due to the increasing requirements of medical imaging applications and the professional requirements of annotating medical images, few-shot learning has gained increasing attention in the medical image semantic segmentation field. To perform segmentation with limited number of labeled medical images, most existing studies use Prototypical Networks (PN) and have obtained compelling success. However, these approaches overlook the query imagefeatures extracted from the proposed representation network, failing to preserving the spatial connection between query and support images. In this paper, we propose a novel self-supervised few-shot medical image segmentation network and introduce a novel Cycle-Resemblance Attention (CRA) module to fully leverage the pixel-wise relation between query and support medical images. Notably, we first line up multiple attention blocks to refine more abundant relation information. Then, we present CRAPNet by integrating the CRA module with a classic prototype network, where pixel-wise relations between query and support features are well recaptured for segmentation. Extensive experiments on two different medical image datasets, e.g., abdomen MRI and abdomen CT, demonstrate the superiority of our model over existing state-of-the-art methods.

SUMMARY: This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. AVAILABILITY AND IMPLEMENTATION: nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Fine-grained activity understanding in videos has attracted considerable recent attention with a shift from action classification to detailed actor and action understanding that provides compelling results for perceptual needs of cutting-edge autonomous systems. However, current methods for detailed understanding of actor and action have significant limitations: they require large amounts of finely labeled data, and they fail to capture any internal relationship among actors and actions. To address these issues, in this paper, we propose a novel, robust multi-task ranking model for weakly supervised actor-action segmentation where only video-level tags are given for training samples. Our model is able to share useful information among different actors and actions while learning a ranking matrix to select representative supervoxels for actors and actions respectively. Final segmentation results are generated by a conditional random field that considers various ranking scores for different video parts. Extensive experimental results on the Actor-Action Dataset (A2D) demonstrate that the proposed approach outperforms the state-of-the-art weakly supervised methods and performs as well as the top-performing fully supervised method.

Reconstruction of neuroanatomy is a fundamental problem in neuroscience. Stochastic expression of colors in individual cells is a promising tool, although its use in the nervous system has been limited due to various sources of variability in expression. Moreover, the intermingled anatomy of neuronal trees is challenging for existing segmentation algorithms. Here, we propose a method to automate the segmentation of neurons in such (potentially pseudo-colored) images. The method uses spatio-color relations between the voxels, generates supervoxels to reduce the problem size by four orders of magnitude before the final segmentation, and is parallelizable over the supervoxels. To quantify performance and gain insight, we generate simulated images, where the noise level and characteristics, the density of expression, and the number of fluorophore types are variable. We also present segmentations of real Brainbow images of the mouse hippocampus, which reveal many of the dendritic segments.