This paper presents a comprehensive digital forensic analysis of the social media platforms Threads and Bluesky, juxtaposing their unique architectures and functionalities against X. This research fills a gap in the extant literature by offering a novel forensic analysis of Threads and Bluesky, based on established techniques. Mobile forensic analysis of both platforms yielded few results. Network analysis produced a variety of artifacts for Bluesky, including plaintext passwords. Threads proved to be robust, and a presentation of its security and API flow is presented. A detailed depiction of the forensic analysis performed for this paper is presented to aid future investigators.

Current paradigms for client-server authentication often rely on username/password schemes. Studies show such schemes are increasingly vulnerable to heuristic and brute-force attacks. This is either due to poor practices by users such as insecure weak passwords, or insecure systems by server operators. A recurring problem in any system which retains information is insecure management policies for sensitive information, such as logins and passwords, by both hosts and users. Increased processing power on the horizon also threatens the security of many popular hashing algorithms. Furthermore, increasing reliance on applications that exchange sensitive information has resulted in increased urgency. This is demonstrated by a large number of mobile applications being deemed insecure by Open Web Application Security Project (OWASP) standards. This paper proposes a secure alternative technique of authentication that retains the current ecosystem, while minimizes attack vectors without inflating responsibilities on users or server operators. Our proposed authentication scheme uses layered encryption techniques alongside a two-part verification process. In addition, it provides dynamic protection for preventing against common cyber-attacks such as replay and man-in-the-middle attacks. Results show that our proposed authentication mechanism outperform other schemes in terms of deployability and resilience to cyber-attacks, without inflating transaction's speed.

BACKGROUND: Metagenomics studies provide valuable insight into the composition and function of microbial populations from diverse environments; however, the data processing pipelines that rely on mapping reads to gene catalogs or genome databases for cultured strains yield results that underrepresent the genes and functional potential of uncultured microbes. Recent improvements in sequence assembly methods have eased the reliance on genome databases, thereby allowing the recovery of genomes from uncultured microbes. However, configuring these tools, linking them with advanced binning and annotation tools, and maintaining provenance of the processing continues to be challenging for researchers. RESULTS: Here we present ATLAS, a software package for customizable data processing from raw sequence reads to functional and taxonomic annotations using state-of-the-art tools to assemble, annotate, quantify, and bin metagenome data. Abundance estimates at genome resolution are provided for each sample in a dataset. ATLAS is written in Python and the workflow implemented in Snakemake; it operates in a Linux environment, and is compatible with Python 3.5+ and Anaconda 3+ versions. The source code for ATLAS is freely available, distributed under a BSD-3 license. CONCLUSIONS: ATLAS provides a user-friendly, modular and customizable Snakemake workflow for metagenome data processing; it is easily installable with conda and maintained as open-source on GitHub at https://github.com/metagenome-atlas/atlas.

BACKGROUND: The Bioinformatics Resource Manager (BRM) is a web-based tool developed to facilitate identifier conversion and data integration for Homo sapiens (human), Mus musculus (mouse), Rattus norvegicus (rat), Danio rerio (zebrafish), and Macaca mulatta (macaque), as well as perform orthologous conversions among the supported species. In addition to providing a robust means of identifier conversion, BRM also incorporates a suite of microRNA (miRNA)-target databases upon which to query target genes or to perform reverse target lookups using gene identifiers. RESULTS: BRM has the capability to perform cross-species identifier lookups across common identifier types, directly integrate datasets across platform or species by performing identifier retrievals in the background, and retrieve miRNA targets from multiple databases simultaneously and integrate the resulting gene targets with experimental mRNA data. Here we use workflows provided in BRM to integrate RNA sequencing data across species to identify common biomarkers of exposure after treatment of human lung cells and zebrafish to benzo[a]pyrene (BAP). We further use the miRNA Target workflow to experimentally determine the role of miRNAs as regulators of BAP toxicity and identify the predicted functional consequences of miRNA-target regulation in our system. The output from BRM can easily and directly be uploaded to freely available visualization tools for further analysis. From these examples, we were able to identify an important role for several miRNAs as potential regulators of BAP toxicity in human lung cells associated with cell migration, cell communication, cell junction assembly and regulation of cell death. CONCLUSIONS: Overall, BRM provides bioinformatics tools to assist biologists having minimal programming skills with analysis and integration of high-content omics' data from various transcriptomic and proteomic platforms. BRM workflows were developed in Java and other open-source technologies and are served publicly using Apache Tomcat at https://cbb.pnnl.gov/brm/ .