This paper presents an extensive statistical analysis of the acoustic startle response of two vocal parameters: fundamental frequency (F0) and root-mean-square energy (E), as well as of the orbicularis oculi (eyeblink) surface electromyography (sEMG). An experiment was conducted in which fourteen participants were exposed to acoustic startle stimuli of varying parameters, i.e., intensity level, duration, rise time, and spectral type, during periods of sustained phonation. Voice recordings of the phonations were taken alongside several physiological signals, of which only the sEMG was analyzed in this paper. Response features (peak value, peak time, latency, rise time, fall time, and duration) were extracted on F0, E and sEMG data, and statistical analysis was conducted using linear mixed effects models to show the response behavior with the varying stimuli. The results for vocal F0 and E data were congruent with sEMG data and earlier work in the field. The results demonstrated that vocal analysis can be used as a feasible alternative to the sEMG eyeblink analysis of acoustic startle responses.