In-vitro fertilization (IVF) has become standard practice to address infertility, which affects more than one in ten couples in the US. However, current protocols yield relatively low success rates of about 20% per treatment cycle. A critical but complex and time-consuming step is the grading and selection of embryos for implantation. Although incubators with time-lapse microscopy have enabled computational analysis of embryo development, existing automated approaches either require extensive manual annotations or use opaque deep learning models that are hard for clinicians to validate and trust. We present EmbryoProfiler, a visual analytics system collaboratively developed with embryologists, biologists, and machine learning researchers to support clinicians in visually assessing embryo viability from time-lapse microscopy imagery. Our system incorporates a deep learning pipeline that automatically annotates microscopy images and extracts clinically interpretable features relevant for embryo grading. Our contributions include: (1) a semi-automatic, visualization-based workflow that guides clinicians through fertilization assessment, developmental timing evaluation, morphological inspection, and comparative analysis of embryos; (2) innovative interactive visualizations, such as cell-shape plots, designed to facilitate efficient analysis of morphological and developmental characteristics; and (3) an integrated, explainable machine learning classifier offering transparent, clinically-informed embryo viability scoring to predict live birth outcomes. Quantitative evaluation of our classifier and qualitative case studies conducted with practitioners demonstrate that EmbryoProfiler enables clinicians to make better-informed embryo selection decisions, potentially leading to improved clinical outcomes in IVF treatments.

We present Cell2Cell, a novel visual analytics approach for quantifying and visualizing networks of cell-cell interactions in three-dimensional (3D) multi-channel cancerous tissue data. By analyzing cellular interactions, biomedical experts can gain a more accurate understanding of the intricate relationships between cancer and immune cells. Recent methods have focused on inferring interaction based on the proximity of cells in low-resolution 2D multi-channel imaging data. By contrast, we analyze cell interactions by quantifying the presence and levels of specific proteins within a tissue sample (protein expressions) extracted from high-resolution 3D multi-channel volume data. Such analyses have a strong exploratory nature and require a tight integration of domain experts in the analysis loop to leverage their deep knowledge. We propose two complementary semi-automated approaches to cope with the increasing size and complexity of the data interactively: On the one hand, we interpret cell-to-cell interactions as edges in a cell graph and analyze the image signal (protein expressions) along those edges, using spatial as well as abstract visualizations. Complementary, we propose a cell-centered approach, enabling scientists to visually analyze polarized distributions of proteins in three dimensions, which also captures neighboring cells with biochemical and cell biological consequences. We evaluate our application in three case studies, where biologists and medical experts use Cell2Cell to investigate tumor micro-environments to identify and quantify T-cell activation in human tissue data. We confirmed that our tool can fully solve the use cases and enables a streamlined and detailed analysis of cell-cell interactions.

In our current visual-centric digital age, the capability to interpret, understand, and produce visual representations of data —termed visualization literacy— is paramount. However, not everyone is adept at navigating this visual terrain. This paper explores the barriers that individuals who misread a visualization encounter, aiming to understand their specific mental gaps.

Trust is an essential aspect of data visualization, as it plays a crucial role in the interpretation and decision-making processes of users. While research in social sciences outlines the multi-dimensional factors that can play a role in trust formation, most data visualization trust researchers employ a single-item scale to measure trust. We address this gap by proposing a comprehensive, multidimensional conceptualization and operationalization of trust in visualization. We do this by applying general theories of trust from social sciences, as well as synthesizing and extending earlier work and factors identified by studies in the visualization field. We apply a two-dimensional approach to trust in visualization, to distinguish between cognitive and affective elements, as well as between visualization and data-specific trust antecedents. We use our framework to design and run a large crowd-sourced study to quantify the role of visual complexity in establishing trust in science visualizations. Our study provides empirical evidence for several aspects of our proposed theoretical framework, most notably the impact of cognition, affective responses, and individual differences when establishing trust in visualizations.

Visual stories are an effective and powerful tool to convey specific information to a diverse public. Scrollytelling is a recent visual storytelling technique extensively used on the web, where content appears or changes as users scroll up or down a page. By employing the familiar gesture of scrolling as its primary interaction mechanism, it provides users with a sense of control, exploration and discoverability while still offering a simple and intuitive interface. In this article, we present a novel approach for authoring, editing, and presenting data-driven scientific narratives using scrollytelling. Our method flexibly integrates common sources such as images, text, and video, but also supports more specialized visualization techniques such as interactive maps as well as scalar field and mesh data visualizations. We show that scrolling navigation can be used to traverse dynamic narratives and demonstrate how it can be combined with interactive parameter exploration. The resulting system consists of an extensible web-based authoring tool capable of exporting stand-alone stories that can be hosted on any web server. We demonstrate the power and utility of our approach with case studies from several diverse scientific fields and with a user study including 12 participants of diverse professional backgrounds. Furthermore, an expert in creating interactive articles assessed the usefulness of our approach and the quality of the created stories.

Tumor tissue characteristics derived from imaging data are gaining importance in clinical research. Tumor sub-regions may play a critical role in defining tumor types and may hold essential information about tumor aggressiveness. Depending on the tumor’s location within the body, such sub-regions can be easily identified and determined by physiology, but these sub-regions are not readily visible to others. Regions within a tumor are currently explored by comparing the image sequences and analyzing the tissue heterogeneity present. To improve the exploration of such tumor sub-regions, we propose a visual analytics tool called ICEVis. ICEVis supports the identification of tumor sub-regions and corresponding features combined with cluster visualizations highlighting cluster validity. It is often difficult to estimate the optimal number of clusters; we provide rich facilities to support this task, incorporating various statistical measures and interactive exploration of the results. We evaluated our tool with three clinical researchers to show the potential of our approach.

Abstract Better understanding of the complex processes driving tumor growth and metastases is critical for developing targeted treatment strategies in cancer. Radiomics extracts large amounts of features from medical images which enables radiomic tumor profiling in combination with clinical markers. However, analyzing complex imaging data in combination with clinical data is not trivial and supporting tools aiding in these exploratory analyses are presently missing. In this paper, we present an approach that aims to enable the analysis of multiparametric medical imaging data in combination with numerical, ordinal, and categorical clinical parameters to validate established and unravel novel biomarkers. We propose a hybrid approach where dimensionality reduction to a single axis is combined with multiple linked views allowing clinical experts to formulate hypotheses based on all available imaging data and clinical parameters. This may help to reveal novel tumor characteristics in relation to molecular targets for treatment, thus providing better tools for enabling more personalized targeted treatment strategies. To confirm the utility of our approach, we closely collaborate with experts from the field of gynecological cancer imaging and conducted an evaluation with six experts in this field.