Photoacoustic tomography (PAT) and magnetic resonance imaging (MRI) are two advanced imaging techniques widely used in pre-clinical research. PAT has high optical contrast and deep imaging range but poor soft tissue contrast, whereas MRI provides excellent soft tissue information but poor temporal resolution. Despite recent advances in medical image fusion with pre-aligned multimodal data, PAT-MRI image fusion remains challenging due to misaligned images and spatial distortion. To address these issues, we propose an unsupervised multi-stage deep learning framework called PAMRFuse for misaligned PAT and MRI image fusion. PAMRFuse comprises a multimodal to unimodal registration network to accurately align the input PAT-MRI image pairs and a self-attentive fusion network that selects information-rich features for fusion. We employ an end-to-end mutually reinforcing mode in our registration network, which enables joint optimization of cross-modality image generation and registration. To the best of our knowledge, this is the first attempt at information fusion for misaligned PAT and MRI. Qualitative and quantitative experimental results show the excellent performance of our method in fusing PAT-MRI images of small animals captured from commercial imaging systems.

As an emerging molecular imaging modality, Photoacoustic Tomography (PAT) is capable of mapping tissue physiological metabolism and exogenous contrast agent information with high specificity. Due to its ultrasonic detection mechanism, the precise localization of targeted lesions has long been a challenge for PAT imaging. The poor soft-tissue contrast of the PAT image makes this process difficult and inaccurate. To meet this challenge, in this study, we first make use of the rich and clear structural information brought about by another advanced imaging modality, Magnetic Resonance Imaging (MRI), to assist organ segmentation and correct for the light fluence attenuation of PAT. We demonstrate improved feature visibility and enhanced localization of endogenous and exogenous agents in the fluence corrected PAT images. Compared with PAT-based methods, the contrast-to-noise ratio (CNR) of our MRI-assisted method increases by 29.1% in live animal experiments. Furthermore, we show that the co-registered MRI image can also be incorporated into PAT image restoration, and achieves improved anatomical landscape and soft-tissue contrast (CNR increased by 25.36%) while preserving similar spatial resolution. This PAT-MRI combination provides excellent structural, functional and molecular images of the subject, and may enable more comprehensive analysis of various preclinical research applications.

The spatial resolution of photoacoustic tomography (PAT) can be characterized by the point spread function (PSF) of the imaging system. Due to the tomographic detection geometry, the PAT image degradation model could be generally described by using spatially variant PSFs. Deconvolution of the PAT image with these PSFs could restore image resolution and recover object details. Previous PAT image restoration algorithms assume that the degraded images can be restored by either a single uniform PSF, or some blind estimation of the spatially variant PSFs. In this work, we propose a PAT image restoration method to improve image quality and resolution based on experimentally measured spatially variant PSFs. Using photoacoustic absorbing microspheres, we design a rigorous PSF measurement procedure, and successfully acquire a dense set of spatially variant PSFs for a commercial cross-sectional PAT system. A pixel-wise PSF map is further obtained by employing a multi-Gaussian-based fitting and interpolation algorithm. To perform image restoration, an optimization-based iterative restoration model with two kinds of regularizations is proposed. We perform phantom and in vivo mice imaging experiments to verify the proposed method, and the results show significant image quality and resolution improvement.

Multispectral photoacoustic tomography (PAT) is capable of resolving tissue chromophore distribution based on spectral un-mixing. It works by identifying the absorption spectrum variations from a sequence of photoacoustic images acquired at multiple illumination wavelengths. Due to multispectral acquisition, this inevitably creates a large dataset. To cut down the data volume, sparse sampling methods that reduce the number of detectors have been developed. However, image reconstruction of sparse sampling PAT is challenging because of insufficient angular coverage. During spectral un-mixing, these inaccurate reconstructions will further amplify imaging artefacts and contaminate the results. To solve this problem, we present the interlaced sparse sampling (ISS) PAT, a method that involved: 1) a novel scanning-based image acquisition scheme in which the sparse detector array rotates while switching illumination wavelength, such that a dense angular coverage could be achieved by using only a few detectors; and 2) a corresponding image reconstruction algorithm that makes use of an anatomical prior image created from the ISS strategy to guide PAT image computation. Reconstructed from the signals acquired at different wavelengths (angles), this self-generated prior image fuses multispectral and angular information, and thus has rich anatomical features and minimum artefacts. A specialized iterative imaging model that effectively incorporates this anatomical prior image into the reconstruction process is also developed. Simulation, phantom, and in vivo animal experiments showed that even under 1/6 or 1/8 sparse sampling rate, our method achieved comparable image reconstruction and spectral un-mixing results to those obtained by conventional dense sampling method.