Biomarker detection is an indispensable part of the diagnosis and treatment of low-grade glioma (LGG). However, current LGG biomarker detection methods rely on expensive and complex molecular genetic testing, for which professionals are required to analyze the results, and intra-rater variability is often reported. To overcome these challenges, we propose an interpretable deep learning pipeline, named Multi-Biomarker Histomorphology Discoverer (Multi-Beholder), to predict the status of five biomarkers in LGG using only hematoxylin and eosin-stained whole slide images. Specifically, Multi-Beholder incorporates one-class classification into the multiple instance learning framework to achieve accurate instance-level pseudo-labeling, thereby complementing slide-level labels and improving prediction performance. Multi-Beholder demonstrates high performance on two LGG cohorts with diverse races and scanning protocols, with area under the receiver operating characteristic curve up to 0.973 on the internal-validated TCGA-LGG dataset and 0.820 on the external-validated Xiangya cohort. Moreover, the interpretability of Multi-Beholder allows for discovering quantitative and qualitative correlations between biomarker status and histomorphology characteristics. Our pipeline not only provides a novel approach for biomarker prediction, enhancing the applicability of molecular treatments for LGG patients but also facilitates the discovery of new mechanisms in molecular functionality and LGG progression. Code can be accessed athttps://github.com/Vison307/Multi-Beholder.

As 3D Gaussian Splatting (3D-GS) emerges as a promising technique for 3D reconstruction and novel view synthesis, offering superior rendering quality and efficiency, it becomes crucial to ensure secure transmission and copyright protection of 3D assets in anticipation of widespread distribution. While steganography has advanced significantly in common 3D media like meshes and Neural Radiance Fields (NeRF), research into steganography for 3D- GS representations remains largely unexplored. To address this gap, we propose ConcealGS, a novel 3D steganography method that embeds implicit information into the explicit 3D representation of Gaussian Splatting. By introducing a consistency strategy for the decoder and a gradient optimization approach, ConcealGS overcomes limitations of NeRF-based models, enhancing both the robustness of implicit information and the quality of 3D reconstruction. Extensive evaluations across various potential application scenarios demonstrate that ConcealGS successfully recovers implicit information with negligible impact on rendering quality, offering a groundbreaking approach for embedding invisible yet recoverable information into 3D models. This work paves the way for advanced copyright protection and secure data transmission in the evolving landscape of 3D content creation and distribution. Code is available at https://github.com/zxk1212/ConcealGS.

In clinical practice, frozen section (FS) images can be utilized to obtain the immediate pathological results of the patients in operation due to their fast production speed. However, compared with the formalin-fixed and paraffin-embedded (FFPE) images, the FS images greatly suffer from poor quality. Thus, it is of great significance to transfer the FS image to the FFPE one, which enables pathologists to observe high-quality images in operation. However, obtaining the paired FS and FFPE images is quite hard, so it is difficult to obtain accurate results using supervised methods. Apart from this, the FS to FFPE stain transfer faces many challenges. Firstly, the number and position of nuclei scattered throughout the image are hard to maintain during the transfer process. Secondly, transferring the blurry FS images to the clear FFPE ones is quite challenging. Thirdly, compared with the center regions of each patch, the edge regions are harder to transfer. To overcome these problems, a multi-perspective self-supervised GAN, incorporating three auxiliary tasks, is proposed to improve the performance of FS to FFPE stain transfer. Concretely, a nucleus consistency constraint is designed to enable the high-fidelity of nuclei, an FFPE guided image deblurring is proposed for improving the clarity, and a multi-field-of-view consistency constraint is designed to better generate the edge regions. Objective indicators and pathologists' evaluation for experiments on the five datasets across different countries have demonstrated the effectiveness of our method. In addition, the validation in the downstream task of microsatellite instability prediction has also proved the performance improvement by transferring the FS images to FFPE ones. Our code link is https://github.com/linyiyang98/Self-Supervised-FS2FFPE.git.

In the era of Artificial Intelligent (AI) and big data, the demand for signal processing algorithms for large datasets has grown across various fields, notably in the application of the Fast Fourier Transform (FFT). This algorithm is crucial in computation and storage-heavy applications like image and radar signal processing, where it has been integral for decades. In-memory computing (IMC), unlike traditional computing models, integrates computation and storage in the same unit, reducing data transfer time and energy costs. This paper presents CSIFA, a hardware accelerator for up to 1024-point FFT algorithm, utilizing SRAM and some other digital logic to enhance efficiency. Our evaluation indicates that CSIFA performing FFT is able to achieve an overall throughput 15MB/s, computational power 6.65mW, and 115.3GOPS, showcasing its high throughput and energy efficiency for edge application scenarios.

Rowhammer has drawn much attention from both academia and industry in the past years as rowhammer exploitation poses severe consequences to system security. Since the first comprehensive study of rowhammer in 2014, a number of rowhammer attacks have been demonstrated against dynamic random access memory (DRAM)-based commodity systems to break software confidentiality, integrity and availability. Accordingly, numerous software defenses have been proposed to mitigate rowhammer attacks on commodity systems of either legacy (e.g., DDR3) or recent DRAM (e.g., DDR4). Besides, multiple hardware defenses (e.g., Target Row Refresh) from the industry have been deployed into recent DRAM to eliminate rowhammer, which we categorize as production defenses.

Different types of staining highlight different structures in organs, thereby assisting in diagnosis. However, due to the impossibility of repeated staining, we cannot obtain different types of stained slides of the same tissue area. Translating the slide that is easy to obtain (e.g., H&E) to slides of staining types difficult to obtain (e.g., MT, PAS) is a promising way to solve this problem. However, some regions are closely connected to other regions, and to maintain this connection, they often have complex structures and are difficult to translate, which may lead to wrong translations. In this paper, we propose the Attention-Based Varifocal Generative Adversarial Network (AV-GAN), which solves multiple problems in pathologic image translation tasks, such as uneven translation difficulty in different regions, mutual interference of multiple resolution information, and nuclear deformation. Specifically, we develop an Attention-Based Key Region Selection Module, which can attend to regions with higher translation difficulty. We then develop a Varifocal Module to translate these regions at multiple resolutions. Experimental results show that our proposed AV-GAN outperforms existing image translation methods with two virtual kidney tissue staining tasks and improves FID values by 15.9 and 4.16 respectively in the H&E-MT and H&E-PAS tasks.

In histopathology, the tissue slides are usually stained by common H&E stain or special stains (MAS, PAS, and PASM, etc.) to clearly show specific tissue structures. The rapid development of deep learning provides a good solution to generate virtual staining images to significantly reduce the time and labor costs associated with histochemical staining. However, most existing methods need to train a special model for every two stains, which consumes a lot of computing resources with the increasing of staining types. To address this problem, we propose an unsupervised multi-domain stain transfer method, GramGAN, which realizes the progressive transfer through cascaded Style-Guided blocks. For each Style-Guided block, we design a style encoding dictionary to characterize and store all the staining style information. In addition, we propose a Rényi entropy-based regularization term to improve the discrimination ability of different styles. The experimental results show that our method can realize accurate transferring among multiple staining styles with better performance. Furthermore, we build and publish a special stained image dataset suitable for glomeruli segmentation (including H&E staining), where the accuracy of glomeruli detection and segmentation can be significantly improved after transferring H&E-stained images to PAS-stained and PASM-stained ones by our method. The code is publicly available at: https://github.com/xianchaoguan/GramGAN.

Polyp segmentation is important in the early diagnosis and treatment of colorectal cancer. Since polyps vary in shape, size, color, and texture, accurate polyp segmentation is very challenging. One promising solution is to model the contextual relation for each pixel. However, previous methods only focus on learning the dependencies between the position within an individual image and ignore the contextual relation across different images. In this paper, we propose a memory-based feature enhancement module to capture the cross-image contextual relations. Specifically, we first present a polyp-centric representation. Then a semantic memory is designed to extract the polyp prototypes across different images. The feature at one position can be further enhanced by the contextual embeddings stored in the semantic memory. The enhanced feature is propagated into the features of the previous levels as the multi-scale guidance. The experimental results show that our method achieves better performance than other state-of-the-art methods.

As an essential step in the pathological diagnosis, histochemical staining can show specific tissue structure information and, consequently, assist pathologists in making accurate diagnoses. Clinical kidney histopathological analyses usually employ more than one type of staining: H&E, MAS, PAS, PASM, etc. However, due to the interference of colors among multiple stains, it is not easy to perform multiple staining simultaneously on one biological tissue. To address this problem, we propose a network based on unpaired training data to virtually generate multiple types of staining from one staining. Our method can preserve the content of input images while transferring them to multiple target styles accurately. To efficiently control the direction of stain transfer, we propose a style guided normalization (SGN). Furthermore, a multiple style encoding (MSE) is devised to represent the relationship among different staining styles dynamically. An improved one-hot label is also proposed to enhance the generalization ability and extendibility of our method. Vast experiments have demonstrated that our model can achieve superior performance on a tiny dataset. The results exhibit not only good performance but also great visualization and interpretability. Especially, our method also achieves satisfactory results over cross-tissue, cross-staining as well as cross-task. We believe that our method will significantly influence clinical stain transfer and reduce the workload greatly for pathologists. Our code and Supplementary materials are available at https://github.com/linyiyang98/UMDST.