Histopathological examination primarily relies on hematoxylin and eosin (H&E) and immunohistochemical (IHC) staining. Though IHC provides more crucial molecular information for diagnosis, it is more costly than H&E staining. Stain transfer technology seeks to efficiently generate virtual IHC images from H&E images. While current deep learning-based methods have made progress, they still struggle to maintain pathological and structural consistency across biomarkers without pixel-level aligned reference. To address the problem, we propose an Auxiliary Task supervision-based Stain Transfer method for multi-biomarkers (ATST-Net), which pioneeringly employs human annotation-free masks as ground truth (GT). ATST-Net ensures pathological consistency, structural preservation and style transfer. It automatically annotates H&E masks in a cost-effective manner by utilizing consecutive IHC sections. Multiple auxiliary tasks provide diverse supervisory information on the location and intensity of biomarker expression, ensuring model accuracy and interpretability. We design a pretrained model-based generator to extract deep feature in H&E images, improving generalization performance. Extensive experiments demonstrate the effectiveness of ATST-Net's components. Compared to existing methods, ATST-Net achieves state-of-the-art (SOTA) accuracy on datasets with multiple biomarkers and intensity levels, while also reflecting high practical value. Code is available at https://github.com/SikangSHU/ATST-Net.

Medical imaging is limited by acquisition time and scanning equipment. CT and MR volumes, reconstructed with thicker slices, are anisotropic with high in-plane resolution and low through-plane resolution. We reveal an intriguing phenomenon that due to the mentioned nature of data, performing slice-wise interpolation from the axial view can yield greater benefits than performing super-resolution from other views. Based on this observation, we propose an Inter-Intra-slice Interpolation Network ( [Formula: see text]Net), which fully explores information from high in-plane resolution and compensates for low through-plane resolution. The through-plane branch supplements the limited information contained in low through-plane resolution from high in-plane resolution and enables continual and diverse feature learning. In-plane branch transforms features to the frequency domain and enforces an equal learning opportunity for all frequency bands in a global context learning paradigm. We further propose a cross-view block to take advantage of the information from all three views online. Extensive experiments on two public datasets demonstrate the effectiveness of [Formula: see text]Net, and noticeably outperforms state-of-the-art super-resolution, video frame interpolation and slice interpolation methods by a large margin. We achieve 43.90dB in PSNR, with at least 1.14dB improvement under the upscale factor of ×2 on MSD dataset with faster inference. Code is available at https://github.com/DeepMed-Lab-ECNU/Medical-Image-Reconstruction.