Protein fitness optimization involves finding a protein sequence that maximizes desired quantitative properties in a combinatorially large design space of possible sequences. Recent advances in steering protein generative models (e.g., diffusion models and language models) with labeled data offer a promising approach. However, most previous studies have optimized surrogate rewards and/or utilized large amounts of labeled data for steering, making it unclear how well existing methods perform and compare to each other in real-world optimization campaigns where fitness is measured through low-throughput wet-lab assays. In this study, we explore fitness optimization using small amounts (hundreds) of labeled sequence-fitness pairs and comprehensively evaluate strategies such as classifier guidance and posterior sampling for guiding generation from different discrete diffusion models of protein sequences. We also demonstrate how guidance can be integrated into adaptive sequence selection akin to Thompson sampling in Bayesian optimization, showing that plug-and-play guidance strategies offer advantages over alternatives such as reinforcement learning with protein language models. Overall, we provide practical insights into how to effectively steer modern generative models for next-generation protein fitness optimization.

The study of social interactions and collective behaviors through multi-agent video analysis is crucial in biology. While self-supervised keypoint discovery has emerged as a promising solution to reduce the need for manual keypoint annotations, existing methods often struggle with videos containing multiple interacting agents, especially those of the same species and color. To address this, we introduce B-KinD-multi, a novel approach that leverages pre-trained video segmentation models to guide keypoint discovery in multi-agent scenarios. This eliminates the need for time-consuming manual annotations on new experimental settings and organisms. Extensive evaluations demonstrate improved keypoint regression and downstream behavioral classification in videos of flies, mice, and rats. Furthermore, our method generalizes well to other species, including ants, bees, and humans, highlighting its potential for broad applications in automated keypoint annotation for multi-agent behavior analysis. Code available under: B-KinD-Multi