This article proposes a confidence-based approach for combining two visual tracking techniques to minimize the influence of unforeseen visual tracking failures to achieve uninterrupted vision-based control. Despite research efforts in vision-guided micromanipulation, existing systems are not designed to overcome visual tracking failures, such as inconsistent illumination condition, regional occlusion, unknown structures, and nonhomogenous background scene. There remains a gap in expanding current procedures beyond the laboratory environment for practical deployment of vision-guided micromanipulation system. A hybrid tracking method, which combines motion-cue feature detection and score-based template matching, is incorporated in an uncalibrated vision-guided workflow capable of self-initializing and recovery during the micromanipulation. Weighted average, based on the respective confidence indices of the motion-cue feature localization and template-based trackers, is inferred from the statistical accuracy of feature locations and the similarity score-based template matches. Results suggest improvement of the tracking performance using hybrid tracking under the conditions. The mean errors of hybrid tracking are maintained at subpixel level under adverse experimental conditions while the original template matching approach has mean errors of 1.53, 1.73, and 2.08 pixels. The method is also demonstrated to be robust in the nonhomogeneous scene with an array of plant cells. By proposing a self-contained fusion method that overcomes unforeseen visual tracking failures using pure vision approach, we demonstrated the robustness in our developed low-cost micromanipulation platform.

Automatic targeting of plant cells to perform tasks like extraction of chloroplast is often desired in the study of plant biology. Hence, this paper proposes an improved cell segmentation method combined with a robust tracking algorithm for vision-guided micromanipulation in plant cells. The objective of this work is to develop an automatic plant cell detection and localization technique to complete the automated workflow for plant cell manipulation. The complex structural properties of plant cells make both segmentation of cells and visual tracking of the microneedle immensely challenging, unlike single animal cell applications. Thus, an improved version of watershed segmentation with adaptive thresholding is proposed to detect the plant cells without the need for staining of the cells or additional tedious preparations. To manipulate the needle to reach the identified centroid of the cells, tracking of the needle tip is required. Visual and motion information from two data sources namely, template tracking and projected manipulator trajectory are combined using score-based normalized weighted averaging to continuously track the microneedle. The selection of trackers is influenced by their complementary nature as the former and latter are individually robust against physical and visual uncertainties, respectively. Experimental results validate the effectiveness of the proposed method by detecting plant cell centroids accurately, tracking the microneedle constantly and reaching the plant cell of interest despite the presence of visual disturbances.

Percutaneous Nephrolithotomy is a recommended treatment method for large kidney stone removal. However, the first and most important step, i.e., getting the percutaneous access to create the tract between the targeted calyx and the flank skin, is challenging as the surgeon is often occupied by several tasks at a given time. Therefore, in this paper, we propose a robotic assisted system that collaborates with the surgeon and provides assistance in order for the surgeons to focus on more critical jobs resulting in better surgical performance. A procedure for this robot including three working stages is described. This procedure allows the surgeon to choose a suitable percutaneous target using an ultrasound probe based on his or her experience and the robot will track the respiratory motion of the target kidney stone and insert the needle automatically after the surgeon releases the probe. Experiments are conducted to demonstrate the procedure with the proposed assisted robot for PCNL.

In this paper, an automatic vision-guided micromanipulation approach to facilitate versatile deployment and portable setup is proposed. This paper is motivated by the importance of micromanipulation and the limitations in existing automation technology in micromanipulation. Despite significant advancements in micromanipulation techniques, there remain bottlenecks in integrating and adopting automation for this application. An underlying reason for the gaps is the difficulty in deploying and setting up such systems. To address this, we identified two important design requirements, namely, portability and versatility of the micromanipulation platform. A self-contained vision-guided approach requiring no complicated preparation or setup is proposed. This is achieved through an uncalibrated self-initializing workflow algorithm also capable of assisted targeting. The feasibility of the solution is demonstrated on a low-cost portable microscope camera and compact actuated microstages. Results suggest subpixel accuracy in localizing the tool tip during initialization steps. The self-focus mechanism could recover intentional blurring of the tip by autonomously manipulating it 95.3% closer to the focal plane. The average error in visual servo is less than a pixel with our depth compensation mechanism showing better maintaining of similarity score in tracking. Cell detection rate in a 1637-frame video stream is 97.7% with subpixels localization uncertainty. Our work addresses the gaps in existing automation technology in the application of robotic vision-guided micromanipulation and potentially contributes to the way cell manipulation is performed.

In this paper, we propose a workflow algorithm for timely tracking of the tool tip during cell manipulation using a template-based approach augmented with low level feature detection. Doing so addresses the problem of adverse influences on template-based tracking during tool-cell interaction while maintaining an efficient track-servo framework. This consideration is important in developing autonomous robotic vision-guided micromanipulators. Our method facilitates vision-guided micromanipulation autonomously without manual interventions even during tool-cell interaction. This is done by decomposing the process to four scenarios that operate on their respective mode. The self-initializing mode is first used to localize and focus a region of interest (ROI) which the tip lies in. Once in focus, the tip is manipulated using a unified visual track-servo template-based approach. A reinitialization mechanism will be triggered to prevent tracking from being interrupted by partial cell occlusion of the tracking ROI. This mechanism uses the self-initializing concept combining motion cue and low-level feature detection to localize the needle tip. Following the reinitialization, we further recover tracking of the needle tip using a mechanism that updates the base template. This adaptive approach ensures uninterrupted tracking even when the cell is interacting with the tool and under deformation. Results demonstrated that with the newly incorporated mechanisms, the localized position improved from an error of more than 50% to less than 10% of the specimen size. When there is no specimen in the scene the new workflow shows no adverse effect on the localization through 270 tracked frames. By incorporating reinitialization and recovery to this workflow algorithm, we hope to initiate the first step towards uncalibrated autonomous vision-guided micromanipulation process.