Nanobodies are a unique class of antibodies with significant therapeutic potential. However, existing nanobody databases often lack standardized data, advanced search capabilities, and integrated analysis tools. We previously developed NanoLAS to address data integration. Here, we present NanoLAS 2.0, a comprehensive update that introduces a multi-condition associative search, an enhanced 3D viewer with molecular docking simulation, an improved sequence viewer, and integrated binding site prediction via NanoBERTa-ASP. The platform features an updated database backend and a redesigned user interface. NanoLAS 2.0 (available at https://www.nanolas2.online) provides a powerful all-in-one platform to accelerate nanobody research.

Recent works on two-stage cross-domain detection have widely explored the local feature patterns to achieve more accurate adaptation results. These methods heavily rely on the region proposal mechanisms and ROI-based instance-level features to design fine-grained feature alignment modules with respect to the foreground objects. However, for one-stage detectors, it is hard or even impossible to obtain explicit instance-level features in the detection pipelines. Motivated by this, we propose an Implicit Instance-Invariant Network (I3Net), which is tailored for adapting one-stage detectors and implicitly learns instance-invariant features via exploiting the natural characteristics of deep features in different layers. Specifically, we facilitate the adaptation from three aspects: (1) Dynamic and Class-Balanced Reweighting (DCBR) strategy, which considers the coexistence of intra-domain and intra-class variations to assign larger weights to those sample-scarce categories and easy-to-adapt samples; (2) Category-aware Object Pattern Matching (COPM) module, which boosts the cross-domain foreground objects matching guided by the categorical information and suppresses the uninformative background features; (3) Regularized Joint Category Alignment (RJCA) module, which jointly enforces the category alignment at different domain-specific layers with a consistency regularization. Experiments reveal that I3Net exceeds the state-of-the-art performance on benchmark datasets.

Domain Adaptive Object Detection (DAOD) relieves the reliance on large-scale annotated data by transferring the knowledge learned from a labeled source domain to a new unlabeled target domain. Recent DAOD approaches resort to local feature alignment in virtue of domain adversarial training in conjunction with the ad-hoc detection pipelines to achieve feature adaptation. However, these methods are limited to adapt the specific types of object detectors and do not explore the cross-domain topological relations. In this paper, we first formulate DAOD as an open-set domain adaptation problem in which foregrounds (pixel or region) can be seen as the “known class”, while backgrounds (pixel or region) are referred to as the “unknown class”. To this end, we present a new and general perspective for DAOD named Dual Bipartite Graph Learning (DBGL), which captures the cross-domain interactions on both pixel-level and semantic-level via increasing the distinction between foregrounds and backgrounds and modeling the cross-domain dependencies among different semantic categories. Experiments reveal that the proposed DBGL in conjunction with one-stage and two-stage detectors exceeds the state-of-the-art performance on standard DAOD benchmarks.

Recent advances in adaptive object detection have achieved compelling results in virtue of adversarial feature adaptation to mitigate the distributional shifts along the detection pipeline. Whilst adversarial adaptation significantly enhances the transferability of feature representations, the feature discriminability of object detectors remains less investigated. Moreover, transferability and discriminability may come at a contradiction in adversarial adaptation given the complex combinations of objects and the differentiated scene layouts between domains. In this paper, we propose a Hierarchical Transferability Calibration Network (HTCN) that hierarchically (local-region/image/instance) calibrates the transferability of feature representations for harmonizing transferability and discriminability. The proposed model consists of three components: (1) Importance Weighted Adversarial Training with input Interpolation (IWAT-I), which strengthens the global discriminability by re-weighting the interpolated image-level features; (2) Context-aware Instance-Level Alignment (CILA) module, which enhances the local discriminability by capturing the underlying complementary effect between the instance-level feature and the global context information for the instance-level feature alignment; (3) local feature masks that calibrate the local transferability to provide semantic guidance for the following discriminative pattern alignment. Experimental results show that HTCN significantly outperforms the state-of-the-art methods on benchmark datasets.