Referring Expression Counting (REC) extends class-level object counting to the fine-grained subclass-level, aiming to enumerate objects matching a textual expression that specifies both the class and distinguishing attribute. A fundamental challenge, however, has been overlooked: annotation points are typically placed on class-representative locations (e.g., heads), forcing models to focus on class-level features while neglecting attribute information from other visual regions (e.g., legs for ''walking''). To address this, we propose W2-Net, a novel framework that explicitly decouples the problem into ''what to count'' and ''where to see'' via a dual-query mechanism. Specifically, alongside the standard what-to-count (w2c) queries that localize the object, we introduce dedicated where-to-see (w2s) queries. The w2s queries are guided to seek and extract features from attribute-specific visual regions, enabling precise subclass discrimination. Furthermore, we introduce Subclass Separable Matching (SSM), a novel matching strategy that incorporates a repulsive force to enhance inter-subclass separability during label assignment. W2-Net significantly outperforms the state-of-the-art on the REC-8K dataset, reducing counting error by 22.5% (validation) and 18.0% (test), and improving localization F1 by 7% and 8%, respectively.

The size of image volumes in connectomics studies now reaches terabyte and often petabyte scales with a great diversity of appearance due to different sample preparation procedures. However, manual annotation of neuronal structures (e.g., synapses) in these huge image volumes is time-consuming, leading to limited labeled training data often smaller than 0.001% of the large-scale image volumes in application. Methods that can utilize in-domain labeled data and generalize to out-of-domain unlabeled data are in urgent need. Although many domain adaptation approaches are proposed to address such issues in the natural image domain, few of them have been evaluated on connectomics data due to a lack of domain adaptation benchmarks. Therefore, to enable developments of domain adaptive synapse detection methods for large-scale connectomics applications, we annotated 14 image volumes from a biologically diverse set of Megaphragma viggianii brain regions originating from three different whole-brain datasets and organized the WASPSYN challenge at ISBI 2023. The annotations include coordinates of pre-synapses and post-synapses in the 3D space, together with their one-to-many connectivity information. This paper describes the dataset, the tasks, the proposed baseline, the evaluation method, and the results of the challenge. Limitations of the challenge and the impact on neuroscience research are also discussed. The challenge is and will continue to be available at https://codalab.lisn.upsaclay.fr/competitions/9169. Successful algorithms that emerge from our challenge may potentially revolutionize real-world connectomics research and further the cause that aims to unravel the complexity of brain structure and function.