Moving infrared small target detection (IRSTD) plays a critical role in practical applications, such as surveillance of unmanned aerial vehicles (UAVs) and UAV-based search system. Moving IRSTD still remains highly challenging due to weak target features and complex background interference. Accurate spatio-temporal feature modeling is crucial for moving target detection, typically achieved through either temporal differences or spatio-temporal (3D) convolutions. Temporal difference can explicitly leverage motion cues but exhibits limited capability in extracting spatial features, whereas 3D convolution effectively represents spatio-temporal features yet lacks explicit awareness of motion dynamics along the temporal dimension. In this paper, we propose a novel moving IRSTD network (TDCNet), which effectively extracts and enhances spatio-temporal features for accurate target detection. Specifically, we introduce a novel temporal difference convolution (TDC) re-parameterization module that comprises three parallel TDC blocks designed to capture contextual dependencies across different temporal ranges. Each TDC block fuses temporal difference and 3D convolution into a unified spatio-temporal convolution representation. This re-parameterized module can effectively capture multi-scale motion contextual features while suppressing pseudo-motion clutter in complex backgrounds, significantly improving detection performance. Moreover, we propose a TDC-guided spatio-temporal attention mechanism that performs cross-attention between the spatio-temporal features extracted from the TDC-based backbone and a parallel 3D backbone. This mechanism models their global semantic dependencies to refine the current frame’s features, thereby guiding the model to focus more accurately on critical target regions. To facilitate comprehensive evaluation, we construct a new challenging benchmark, IRSTD-UAV, consisting of 15,106 real infrared images with diverse low signal-to-clutter ratio scenarios and complex backgrounds. Extensive experiments on IRSTD-UAV and public infrared datasets demonstrate that our TDCNet achieves state-of-the-art detection performance in moving target detection.