A versatile compact power-amplifier cell for rapid development of V-band GaAs MMICs is reported. It was designed using a single-finger, non-linear model with an EM simulation of the interconnect. In a small active area of 1.8 mm2, the cell offers 30.1 dB power gain over the 58–66 GHz band. The versatility for rapid development is demonstrated by assembling various amplifiers: two cells in parallel, a full-balanced amplifier with four cells, and a transceiver MMIC using a single-cell as the transmitter and a half-cell as the receiver. For these variants output power is 27.3, 29.3, 19.6 dBm at 1-dB gain compression, and 29.8, 29.9, 27.3 dBm in saturation respectively.

Abstract Motivation: Deep profiling the phenotypic landscape of tissues using high-throughput flow cytometry (FCM) can provide important new insights into the interplay of cells in both healthy and diseased tissue. But often, especially in clinical settings, the cytometer cannot measure all the desired markers in a single aliquot. In these cases, tissue is separated into independently analysed samples, leaving a need to electronically recombine these to increase dimensionality. Nearest-neighbour (NN) based imputation fulfils this need but can produce artificial subpopulations. Clustering-based NNs can reduce these, but requires prior domain knowledge to be able to parameterize the clustering, so is unsuited to discovery settings. Results: We present flowBin, a parameterization-free method for combining multitube FCM data into a higher-dimensional form suitable for deep profiling and discovery. FlowBin allocates cells to bins defined by the common markers across tubes in a multitube experiment, then computes aggregate expression for each bin within each tube, to create a matrix of expression of all markers assayed in each tube. We show, using simulated multitube data, that flowType analysis of flowBin output reproduces the results of that same analysis on the original data for cell types of >10% abundance. We used flowBin in conjunction with classifiers to distinguish normal from cancerous cells. We used flowBin together with flowType and RchyOptimyx to profile the immunophenotypic landscape of NPM1-mutated acute myeloid leukemia, and present a series of novel cell types associated with that mutation. Availability and implementation: FlowBin is available in Bioconductor under the Artistic 2.0 free open source license. All data used are available in FlowRepository under accessions: FR-FCM-ZZYA, FR-FCM-ZZZK and FR-FCM-ZZES. Contact: [email protected]. Supplementary information: Supplementary data are available at Bioinformatics online.