The treatment of malaria is a global health challenge that stands to benefit from the widespread introduction of a vaccine for the disease. A method has been developed to create a live organism vaccine using the sporozoites (SPZ) of the parasite Plasmodium falciparum (Pf), which are concentrated in the salivary glands of infected mosquitoes. Current manual dissection methods to obtain these PfSPZ are not optimally efficient for large-scale vaccine production. We propose an improved dissection procedure and a mechanical fixture that increases the rate of mosquito dissection and helps to deskill this stage of the production process. We further demonstrate the automation of a key step in this production process, the picking and placing of mosquitoes from a staging apparatus into a dissection assembly. This unit test of a robotic mosquito pick-and-place system is performed using a custom-designed micro-gripper attached to a four degree of freedom (4-DOF) robot under the guidance of a computer vision system. Mosquitoes are autonomously grasped and pulled to a pair of notched dissection blades to remove the head of the mosquito, allowing access to the salivary glands. Placement into these blades is adapted based on output from computer vision to accommodate for the unique anatomy and orientation of each grasped mosquito. In this pilot test of the system on 50 mosquitoes, we demonstrate a 100% grasping accuracy and a 90% accuracy in placing the mosquito with its neck within the blade notches such that the head can be removed. This is a promising result for this difficult and non-standard pick-and-place task.

This work presents the design and evaluation of a low-cost tri-axial force sensor, that has been developed to regain the sense of touch in minimally invasive surgeries (MIS). The force sensor uses an array of force sensitive resistors (FSR) with a mechanically pre-loaded structure to perform the force sensing. The sensor has a built-in signal conditioning circuitry to provide on-board power regulation, programmable signal amplification and analog to digital conversion. The sensor is inexpensive and highly sensitive to low-amplitude force, critical in surgical applications. We validate the efficacy of the sensor with two surgical applications - robotic palpation for stiffness mapping and obstacle avoidance for a highly articulated robotic probe (HARP). The results show that the sensor is capable of accurately detecting the stiff inclusions embedded in the tissues as well as detecting obstacles and helping HARP safely navigate around them.