In resistance training, trainers employ touch guidance to help trainees control posture and activate muscles. Haptic feedback can extend this support to solitary workouts, but translating the nuances of touch into effective haptic patterns remains challenging. In this paper, we categorize the instructional messages conveyed through trainers’ touch guidance and design electrical stimulation patterns to replicate them. A preliminary study with six trainers and six trainees identified six core messages underlying touch guidance. We then designed electrical stimulation patterns for each message and refined them with two sports scientists and a UX designer, ensuring usability and grounding. Finally, sixteen gymgoers evaluated these patterns in a controlled exercise task. Participants reliably distinguished the feedback and used the instructed muscles accordingly, achieving accuracies of 97.14% and 99.22% across two sessions, cross-checked with EMG and pose estimation. These findings demonstrate that the proposed electrical stimulation feedback is intuitive and learnable.

BACKGROUND: Pooled library screen analysis using shRNAs or CRISPR-Cas9 hold great promise to genome-wide functional studies. While pooled library screens are effective tools, erroneous barcodes can potentially be generated during the production of many barcodes. However, no current tools can distinguish erroneous barcodes from PCR or sequencing errors in a data preprocessing step. RESULTS: We developed the Barcas program, a specialized program for the mapping and analysis of multiplexed barcode sequencing (barcode-seq) data. For fast and efficient mapping, Barcas uses a trie data structure based imperfect matching algorithm which generates precise mapping results containing mismatches, shifts, insertions and deletions (indel) in a flexible manner. Barcas provides three functions for quality control (QC) of a barcode library and distinguishes erroneous barcodes from PCR or sequencing errors. It also provides useful functions for data analysis and visualization. CONCLUSIONS: Barcas is an all-in-one package providing useful functions including mapping, data QC, library QC, statistical analysis and visualization in genome-wide pooled screens.