Autoethnographic and other first-person research methods are a topic of increasing interest in design and HCI. This focus parallels the boom in self-tracking and personal informatics, perhaps most intriguingly in the intersection of quantitative and qualitative data and the noticing of patterns in one's own life and everyday wellbeing. But how can design support this? One opportunity is for research probes, or tools, which enable forms of self-inquiry, by design researchers themselves, or others. In this paper-with the broad scope of healthier student sleep as a domain-we present a series of artifacts designed by undergraduates as tools to enable autoethnographic exploration, and detail how they have been used to investigate bedtime routines, personal scheduling of time, focus, sleep data, and sleeping in non-traditional places. We also reflect on the notion of combination autoethnographic 'kits' as a way forward for forms of self-inquiry.

MOTIVATION: PSORTb v.1.1 is the most precise bacterial localization prediction tool available. However, the program's predictive coverage and recall are low and the method is only applicable to Gram-negative bacteria. The goals of the present work are as follows: increase PSORTb's coverage while maintaining the existing precision level, expand it to include Gram-positive bacteria and then carry out a comparative analysis of localization. RESULTS: An expanded database of proteins of known localization and new modules using frequent subsequence-based support vector machines was introduced into PSORTb v.2.0. The program attains a precision of 96% for Gram-positive and Gram-negative bacteria and predictive coverage comparable to other tools for whole proteome analysis. We show that the proportion of proteins at each localization is remarkably consistent across species, even in species with varying proteome size. AVAILABILITY: Web-based version: http://www.psort.org/psortb. Standalone version: Available through the website under GNU General Public License. CONTACT: [email protected], [email protected] SUPPLEMENTARY INFORMATION: http://www.psort.org/psortb/supplementaryinfo.html.