MOTIVATION: Transposable elements (TEs) in eukaryotes often get inserted into one another, forming sequences that become a complex mixture of full-length elements and their fragments. The reconstruction of full-length elements and the order in which they have been inserted is important for genome and transposon evolution studies. However, the accumulation of mutations and genome rearrangements over evolutionary time makes this process error-prone and decreases the efficiency of software aiming to recover all nested full-length TEs. RESULTS: We created software that uses a greedy recursive algorithm to mine increasingly fragmented copies of full-length LTR retrotransposons in assembled genomes and other sequence data. The software called TE-greedy-nester considers not only sequence similarity but also the structure of elements. This new tool was tested on a set of natural and synthetic sequences and its accuracy was compared to similar software. We found TE-greedy-nester to be superior in a number of parameters, namely computation time and full-length TE recovery in highly nested regions. AVAILABILITY AND IMPLEMENTATION: http://gitlab.fi.muni.cz/lexa/nested. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Accurate and reliable visualization of blood vessels is still a challenging problem, notably in the presence of morphologic changes resulting from atherosclerotic diseases. We take advantage of partially segmented data with approximately identified vessel centerlines to comprehensively visualize the diseased peripheral arterial tree. We introduce the VesselGlyph as an abstract notation for novel focus & context visualization techniques of tubular structures such as contrast-medium enhanced arteries in CT-angiography (CTA). The proposed techniques combine direct volume rendering (DVR) and curved planar reformation (CPR) within a single image. The VesselGlyph consists of several regions where different rendering methods are used. The region type, the used visualization method and the region parameters depend on the distance from the vessel centerline and on viewing parameters as well. By selecting proper rendering techniques for different regions, vessels are depicted in a naturally looking and undistorted anatomic context. This may facilitate the diagnosis and treatment planning of patients with peripheral arterial occlusive disease. In this paper we furthermore present a way of how to implement the proposed techniques in software and by means of modern 3D graphics accelerators.