MOTIVATION: Live-cell imaging-based drug screening increases the likelihood of identifying effective and safe drugs by providing dynamic, high-content, and physiologically relevant data. As a result, it improves the success rate of drug development and facilitates the translation of benchside discoveries to bedside applications. Despite these advantages, no comprehensive metrics currently exist to evaluate dose-time-dependent drug responses. To address this gap, we established a systematic framework to assess drug effects across a range of concentrations and exposure durations simultaneously. This metric enables more accurate evaluation of drug responses measured by live-cell imaging. RESULTS: We employed treatment concentrations ranging from 0 to 10 μM and performed live-cell imaging-based measurements over a 120-h incubation period. To analyze the experimental data, we developed VUScope, a new mathematical model combining the 4-parameter logistic curve and a logistic function to characterize dose-time-dependent responses. This enabled us to calculate the Growth Rate Inhibition Volume Under the dose-time-response Surface (GRIVUS), which serves as a critical metric for assessing dynamic drug responses. Furthermore, our mathematical model allowed us to predict long-term treatment responses based on short-term drug responses. We validated the predictive capabilities of our model using independent datasets and observed that VUScope enhances prediction accuracy and offers deeper insights into drug effects than previously possible. By integrating VUScope into high-throughput drug screening platforms, we can further improve the efficacy of drug development and treatment selection. AVAILABILITY AND IMPLEMENTATION: We have made VUScope more accessible to users conducting pharmacological studies by uploading a detailed description, example datasets, and the source code to vuscope.albi.hhu.de, https://github.com/AlBi-HHU/VUScope, and https://doi.org/10.5281/zenodo.17610533.

Semblance velocity analysis plays an indispensable role in seismic data processing. In order to avoid the huge time-cost when performed manually, some deep learning methods are proposed for automatic velocity picking from semblance. However, the application of existing deep learning methods is still restricted by the shortage of labels in practice. To solve this problem, we take semblance velocity analysis as a point-to-point regression problem at each time sample. A time window on semblance which can extract the block corresponding to a time-velocity (t-v) pair and the reference velocity curve (RVC) which can transform semblance randomly are employed together to augment the labeled data. We divide the development of data augmentation strategy into three progressive modes. The datasets from three modes are prepared for training designed attention neural network. The field experiments show that the attention neural network can produce reasonable results and the data augmentation strategy can effectively improve the velocity picking accuracy.

In synthetic aperture sonar (SAS) image, the underwater shipwreck targets are often buried by sediment or badly damaged. Only a part of the characteristics of artificial objects is retained. In this paper, firstly, based on the analysis of the ocean buried background, the Meanshift filtering is used to smooth the original image and convert the color image into binary one. Secondly, the residual contour of artificial target is extracted through the modified Canny edge detection algorithm. Thirdly, the Region Growing method is taken to remove the discrete interference and keep the intact edge of the line. Consideration with the principle of line alignment, the contours of shipwreck targets are gradually connected and aggregated. Finally, a large amount of measured practical SAS images are tested. The experimental results verified that the proposed algorithm can accurately detect the shipwreck target based on residual contour information, meanwhile with an acceptable timeliness for large size sonar image data.

Multi-tiered geo-distributed cloud storage systems must tame complexity at many levels: uniform APIs for storage access, supporting flexible storage policies that meet a wide array of application metrics, determining an optimal data placement, handling uncertain network dynamics and access dynamism, and operating across many levels of heterogeneity both within and across data-centers (DCs). In this paper, we present an integrated solution called Wiera. Wiera enables the specification of data management policies both within a local DC and across DCs. Such policies enable the user to optimize for cost, performance, reliability, durability, and consistency, and to express their tradeoffs. In addition, Wiera determines an optimal data placement for the user to meet their desired tradeoffs easily in such an environment. A key aspect of Wiera is first-class support for dynamism due to network, workload, and access patterns changes. As far as we know, Wiera is the first geo-distributed cloud storage system which handles dynamism actively at run-time. Wiera allowsunmodified applicationsto reap the benefits of flexible data/storage policies by externalizing the policy specification. We show how Wiera enables a rich specification of dynamic policies using a concise notation and describe the design and implementation of the system. We have implemented a Wiera prototype on multiple cloud environments, AWS and Azure, that illustrates potential benefits from managing dynamics and in using multiple cloud storage tiers both within and across DCs.

Existing graphical passwords require users to proactively memorize their secrets and meanwhile these schemes are vulnerable to shoulder surfing attacks. We propose a novel graphical password scheme, PassApp, which utilizes users' everyday memory about installed apps on mobile devices as shared secrets. As the registration stage is no longer needed, PassApp exempts users from additional memory burden and greatly enhances user experience. Additionally, PassApp owns a large password set and only a small part of passwords may be exposed during a login. Therefore, PassApp has a natural advance on effectively resisting guessing attacks and shoulder surfing attacks. Our user studies demonstrate that PassApp performs well with a reasonable login time (7.27s) and a high success rate (95.48%). Our security analysis shows PassApp can effectively withstand one-time shoulder surfing attacks and on average 30 times of shoulder surfing are necessary to expose all passwords.