Objective: Whether it is manual suture or robotic automatic suture, the suture parameters of the kidney directly determine the recovery effect of the wound. However, there is a lack of quantitative research. The purpose of this paper is to reveal the influence of the different suture methods on the effect of the suture operation. Methods: Firstly, an editable three-dimensional model of the kidney was established using CT images. Subsequently, wounds were incised on the surface of the kidney entity and sutures were embedded. Secondly, the ABAQUS software was utilized to conduct a simulation analysis of the suturing process. Thirdly, experiments on the ex vivo porcine renal specimens were sutured manually in parallel to the simulation analysis. Meanwhile, the different tensile forces, under suture intervals, various wound conditions, and suturing methods, were measured to derive the tensile force values. Finally, a comparative study of finite element analysis and specimen experiments was performed. Results: The results showed that the 5 mm suture interval and the internal figure-of-eight suture are the optimal choice for suture and the suture of circular wounds is more difficult than elliptical wounds and rectangular wounds, the tensile force is around 0.3-0.5 N. Conclusion: This paper presents the variation law of the tensile force for different wounds and provides the optimal suturing method for both manual and robotic autonomous suturing. Significance: By revealing the influence of different suture methods and providing the optimal suturing method, it can contribute to improving the suture operation effect and promoting the development of suture technology.