Super-resolution ultrasound (SRUS) imaging through localisation and tracking of microbubble (MB), also known as ultrasound localisation microscopy (ULM), allows non-invasive imaging of microvasculature in vivo beyond the diffraction limit. The number of MBs localised from the acquired contrast-enhanced ultrasound (CEUS) images and the localisation accuracy precision directly influence the quality of the resulting super-resolution microvasculature images. However, non-negligible noise present in the CEUS images can make localising MBs challenging. To enhance the MB localisation performance, we propose a Multi-Frame Deconvolution (MF-Decon) framework that can exploit the spatiotemporal consistency inherent in the CEUS data, with new spatial and temporal regularisers designed based on total variation (TV) and regularisation by denoising (RED). Based on the MF-Decon framework, we introduce two novel methods: MF-Decon with spatial and temporal TVs (MF-Decon+3DTV) and MF-Decon with spatial RED and temporal TV (MF-Decon+RED+TV). Results from in silico simulations indicate that our methods outperform two widely used methods using deconvolution or normalised cross-correlation across all evaluation metrics, including precision, recall, F 1 score, mean and standard localisation errors. In particular, our methods improve MB localisation precision by up to 39% and recall by up to 12%. Super-resolution microvasculature maps generated with our methods on a publicly available in vivo rat brain dataset show less noise, better contrast, higher resolution and more vessel structures.

Super-Resolution Ultrasound (SRUS) imaging through localising and tracking microbubbles, also known as Ultrasound localization Microscopy (ULM), has demonstrated reconstruction of microvascular structure and flow with sub-diffraction resolution, and its potential in a range of clinical applications. However, imaging organs with large tissue movements, such as those caused by respiration, presents substantial challenges. Existing methods often require breath holding to maintain accumulation accuracy, which limits data acquisition time and ULM image saturation. To improve image quality in the presence of large tissue movements, this study introduces an approach integrating high-frame-rate volumetric ultrasound with online precise robotic probe control. Tested on a microvasculature phantom with slow but large translation motions, up to 5 mm/s in speed and 20 mm in distance- twice the aperture size of the matrix array used, our method achieved real-time tracking of the moving phantom and imaging volume rate at 85 Hz, keeping majority of the target volume in the imaging field of view. ULM images of the moving cross channels in the phantom were successfully reconstructed in post-processing, demonstrating the feasibility of super-resolution imaging under large tissue motions. This represents a significant step towards ULM imaging of organs with large motion.

With the widespread interest and uptake of super-resolution ultrasound (SRUS) through localization and tracking of microbubbles, also known as ultrasound localization microscopy (ULM), many localization and tracking algorithms have been developed. ULM can image many centimeters into tissue in-vivo and track microvascular flow non-invasively with sub-diffraction resolution. In a significant community effort, we organized a challenge, Ultrasound Localization and TRacking Algorithms for Super-Resolution (ULTRA-SR). The aims of this paper are threefold: to describe the challenge organization, data generation, and winning algorithms; to present the metrics and methods for evaluating challenge entrants; and to report results and findings of the evaluation. Realistic ultrasound datasets containing microvascular flow for different clinical ultrasound frequencies were simulated, using vascular flow physics, acoustic field simulation and nonlinear bubble dynamics simulation. Based on these datasets, 38 submissions from 24 research groups were evaluated against ground truth using an evaluation framework with six metrics, three for localization and three for tracking. In-vivo mouse brain and human lymph node data were also provided, and performance assessed by an expert panel. Winning algorithms are described and discussed. The publicly available data with ground truth and the defined metrics for both localization and tracking present a valuable resource for researchers to benchmark algorithms and software, identify optimized methods/software for their data, and provide insight into the current limits of the field. In conclusion, Ultra-SR challenge has provided benchmarking data and tools as well as direct comparison and insights for a number of the state-of-the art localization and tracking algorithms.

Perfusion by the microcirculation is key to the development, maintenance and pathology of tissue. Its measurement with high spatiotemporal resolution is consequently valuable but remains a challenge in deep tissue. Ultrasound Localization Microscopy (ULM) provides very high spatiotemporal resolution but the use of microbubbles requires low contrast agent concentrations, a long acquisition time, and gives little control over the spatial and temporal distribution of the microbubbles. The present study is the first to demonstrate Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM) and fast-AWSALM for in vivo super-resolution ultrasound imaging, offering contrast on demand and vascular selectivity. Three different formulations of acoustically activatable contrast agents were used. We demonstrate their use with ultrasound mechanical indices well within recommended safety limits to enable fast on-demand sparse activation and destruction at very high agent concentrations. We produce super-localization maps of the rabbit renal vasculature with acquisition times between 5.5 s and 0.25 s, and a 4-fold improvement in spatial resolution. We present the unique selectivity of AWSALM in visualizing specific vascular branches and downstream microvasculature, and we show super-localized kidney structures in systole (0.25 s) and diastole (0.25 s) with fast-AWSALM outperforming microbubble based ULM. In conclusion, we demonstrate the feasibility of fast and selective imaging of microvascular dynamics in vivo with subwavelength resolution using ultrasound and acoustically activatable nanodroplet contrast agents.

Though physiological signal based human-machine interfaces (HMIs) have recently developed rapidly, their practical use is restricted by many real-world environmental factors, one of which is muscle fatigue. This paper explores the sensitivities between surface electromyography (sEMG) and A-mode ultrasound (AUS) sensing modalities subject to muscle fatigue in the context of hand gesture recognition tasks. Two metrics, mean classification accuracy ( mCA) and decline rate ( DR), are proposed to evaluate the accuracy and muscle fatigue sensitivity between sEMG and AUS based HMIs. Muscle fatigue inducing experiment was designed and eight subjects were recruited to participate in the experiment. The gesture recognition accuracies of sEMG and AUS under non-fatigue state and fatigue state are compared through Mahalanobis distance based classifier linear discriminant analysis (LDA). In addition, Mahalanobis distance based metrics, repeatability index ( RI) and separability index ( SI), are introduced to evaluate the changes in the feature distribution during muscle fatigue and reveal the cause of the fatigue sensitivity difference between sEMG and AUS signals. The experimental results demonstrate that the fatigue robustness of AUS signal is better than that of sEMG signal. Specifically, with the employment of the LDA classifier trained under non-fatigue state, the testing accuracy of the sEMG signal on the non-fatigue state is 94.96%, while reduce to 68.26% on the fatigue state. The testing accuracy of the AUS signal on the corresponding states is 99.68% and 91.24% respectively. AUS signal attains higher mCA and lower DR, indicating that it has advantages over sEMG signal in terms of both accuracy and muscle fatigue sensitivity. In addition, the RI and RI/SI analysis reveal that before and after muscle fatigue, the consistency of AUS feature distribution is better than that of sEMG. These research outcomes validate that AUS is more tolerant to feature migration caused by muscle fatigue than sEMG.

Ultrasound super-resolution imaging through localisation and tracking of microbubbles can achieve sub-wave-diffraction resolution in mapping both micro-vascular structure and flow dynamics in deep tissue in vivo. Currently, it is still challenging to achieve high accuracy in localisation and tracking particularly with limited imaging frame rates and in the presence of high bubble concentrations. This study introduces microbubble image features into a Kalman tracking framework, and makes the framework compatible with sparsity-based deconvolution to address these key challenges. The performance of the method is evaluated on both simulations using individual bubble signals segmented from in vivo data and experiments on a mouse brain and a human lymph node. The simulation results show that the deconvolution not only significantly improves the accuracy of isolating overlapping bubbles, but also preserves some image features of the bubbles. The combination of such features with Kalman motion model can achieve a significant improvement in tracking precision at a low frame rate over that using the distance measure, while the improvement is not significant at the highest frame rate. The in vivo results show that the proposed framework generates SR images that are significantly different from the current methods with visual improvement, and is more robust to high bubble concentrations and low frame rates.

Noninvasive monitoring of muscle contraction, which provides information about muscle morphological deformations, has a great potential in medical applications, such as stroke rehabilitation and prosthesis control. This paper presents a wearable multichannel A-mode ultrasound system for the multiperspective muscle contraction detection against its existing bulky ultrasound sensing counterpart. The system consists of a waveform generator and a waveform amplifier for ultrasound excitation, as well as a signal processing module for echo receiving, amplifying, and transmitting. In addition, a miniaturized transducer was optimized for muscle contraction monitoring using 1-3 piezoelectric composite. The system's superiorities on excitation pulse, detection depth, and axial resolution were validated by the evaluation experiments. And in vivo muscle deformation detection and virtual prosthesis control experiments (target achievement control test) demonstrated its ability in rehabilitation applications, with a task completion rate of 100% and path efficiency of 93.30%. These results confirmed the reliability of the proposed ultrasound system and paved the way for its applications in rehabilitation treatment and prosthesis research.