Collagen self-assembly supports its mechanical function, but controlling collagen mimetic peptides (CMPs) to self-assemble into higher-order oligomers with numerous functions remains challenging due to the vast potential amino acid sequence space. Herein, we developed a diffusion model to learn features from different types of human collagens and generate CMPs; obtaining 66% of synthetic CMPs could self-assemble into triple helices. Triple-helical and untwisting states were probed by melting temperature (Tm); hence, we developed a model to predict collagen Tm, achieving a state-of-art Pearson's correlation (PC) of 0.95 by cross-validation and a PC of 0.8 for predicting Tm values of synthetic CMPs. Our chemically synthesized short CMPs and recombinantly expressed long CMPs could self-assemble, with the lowest requirement for hydrogel formation at a concentration of 0.08% (w/v). Five CMPs could promote osteoblast differentiation. Our results demonstrated the potential for using computer-aided methods to design functional self-assembling CMPs.

Engineering enzyme-substrate binding pockets is the most efficient approach for modifying catalytic activity, but is limited if the substrate binding sites are indistinct. Here, we developed a 3D convolutional neural network for predicting protein-ligand binding sites. The network was integrated by DenseNet, UNet, and self-attention for extracting features and recovering sample size. We attempted to enlarge the dataset by data augmentation, and the model achieved success rates of 48.4%, 35.5%, and 43.6% at a precision of ≥50% and 52%, 47.6%, and 58.1%. The distance of predicted and real center is ≤4 Å, which is based on SC6K, COACH420, and BU48 validation datasets. The substrate binding sites of Klebsiella variicola acid phosphatase (KvAP) and Bacillus anthracis proline 4-hydroxylase (BaP4H) were predicted using DUnet, showing high competitive performance of 53.8% and 56% of the predicted binding sites that critically affected the catalysis of KvAP and BaP4H. Virtual saturation mutagenesis was applied based on the predicted binding sites of KvAP, and the top-ranked 10 single mutations contributed to stronger enzyme-substrate binding varied while the predicted sites were different. The advantage of DUnet for predicting key residues responsible for enzyme activity further promoted the success rate of virtual mutagenesis. This study highlighted the significance of correctly predicting key binding sites for enzyme engineering.

Low-power wide-area networks (LPWAN) evolve rapidly with advanced communication primitives (e.g., coding, modulation) being continuously invented. This rapid iteration on LPWAN, however, forms a communication barrier between legacy wireless sensor nodes deployed years ago (e.g., ZigBee-based sensor node) with their latest competitor running a different communication protocol (e.g., LoRa-based IoT node): they work on the same frequency band but share different MAC- and PHY-layer regulations and thus cannot talk to each other directly. To break this barrier, we propose LigBee, a cross-technology communication (CTC) solution that enables symbol-level communication from the latest LPWAN LoRa node to legacy ZIGBEE node. We have implemented LigBee on both software-defined radios and commercial-off-the-shelf (COTS) LoRa and ZigBee nodes, and demonstrated that LigBee builds a reliable CTC link from LoRa node to ZigBee node on both platforms. Our experimental results show that i) LigBee achieves a bit error rate (BER) in the order of 10−3with 70 ∼ 80% frame reception ratio (FRR), ii) the range of LigBee link is over 300m, which is 6 ∼ 7.5× the typical range of legacy ZigBee and state-of-the-art solution, and iii) the throughput of LigBee link is maintained on the order of kbps, which is close to the LoRa’s throughput.

Recent advances in traffic signal control have witnessed the success of reinforcement learning. However, most of these approaches have focused on vehicle traffic and lack consideration for pedestrians. This can be attributed in part to the fact that the existing underlying technologies are not yet practical to deploy in real-world environments. Vision technologies, for example, can easily be obscured from view in reality. The direction of movement and position of pedestrians is difficult to estimate accurately. The emergence of 6G localization and tracking services offer new opportunities. With this base service, we intend to improve the efficiency, safety, and scalability of multi-intersection traffic signal control with mixed traffic flows. This problem is challenging for its coordination, scalability, and access of new traffic. To solve these challenges, we propose PV-TSC, a distributed reinforcement learning motivated traffic signal control with pedestrian access. We analyze different behaviors of pedestrian traffic, and integrate pedestrian traffic with the proven traffic signal control scheme for vehicle traffic. Finally, we conduct simulation experiments to illustrate the superiority of PV-TSC against classic methods, and further analyze the effectiveness of PV-TSC design by exploring its variants.

Long Range (LoRa) communication, thanks to its wide network coverage and low energy operation, has attracted extensive attentions from both academia and industry. However, existing LoRa-based Wide Area Network (LoRaWAN) suffers from severe inter-network interference, due to the following two reasons. First, the densely-deployed LoRa ends usually share the same network configurations, such as spreading factor (SF), bandwidth (BW) and carrier frequency (CF), causing interference when operating in the vicinity. Second, LoRa is tailored for low-power devices, which excludes LoRaWAN from using the listen-before-talk (LBT) mechanisms commonly used in wireless communication technologies, such as WiFi and ZigBee -LoRaWAN has to use the duty-cycled medium access policy and thus being incapable of channel sensing or collision avoidance. To mitigate the inter-network interference, we propose a novel solution achieving the online concurrent transmissions at LoRa gateway, called OCT, which recovers collided packets at the gateway and thus improves LoRaWAN's throughput. Moreover, OCT achieves the online concurrent transmission using only LoRa's (de)modulation information, thus can be easily deployed at LoRa gateway. We have implemented and evaluated OCT on USRP platform and commodity LoRa ends, showing OCT achieves: (i) >90% packet reception rate (PRR), (ii) 3 × 10-3bit error rate (BER), (iii) 2x and 3x throughput in the scenarios of two- and three- packet collisions respectively, and (iv) reducing 67% latency compared with state-of-the-art.