Acoustic resolution photoacoustic microscopy (AR-PAM) is a novel medical imaging modality, which can be used for both structural and functional imaging in deep bio-tissue. However, the imaging resolution is degraded and structural details are lost since its dependency on acoustic focusing, which significantly constrains its scope of applications in medical and clinical scenarios. To address the above issue, model-based approaches incorporating traditional analytical prior terms have been employed, making it challenging to capture finer details of anatomical bio-structures. In this paper, we proposed an innovative prior named group sparsity prior for simultaneous reconstruction, which utilizes the non-local structural similarity between patches extracted from internal AR-PAM images. The local image details and resolution are improved while artifacts are also introduced. To mitigate the artifacts introduced by patch-based reconstruction methods, we further integrate an external image dataset as an extra information provider and consolidate the group sparsity prior with a deep denoiser prior. In this way, complementary information can be exploited to improve reconstruction results. Extensive experiments are conducted to enhance the simulated and in vivo AR-PAM imaging results. Specifically, in the simulated images, the mean peak signal-to-noise ratio (PSNR) and structural similarity index measure (SSIM) values have increased from 16.36 dB and 0.46 to 27.62 dB and 0.92, respectively. The in vivo reconstructed results also demonstrate the proposed method achieves superior local and global perceptual qualities, the metrics of signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR) have significantly increased from 10.59 and 8.61 to 30.83 and 27.54, respectively. Additionally, reconstruction fidelity is validated with the optical resolution photoacoustic microscopy (OR-PAM) data as reference image.

Acoustic resolution photoacoustic micros- copy (AR-PAM) can achieve deeper imaging depth in biological tissue, with the sacrifice of imaging resolution compared with optical resolution photoacoustic microscopy (OR-PAM). Here we aim to enhance the AR-PAM image quality towards OR-PAM image, which specifically includes the enhancement of imaging resolution, restoration of micro-vasculatures, and reduction of artifacts. To address this issue, a network (MultiResU-Net) is first trained as generative model with simulated AR-OR image pairs, which are synthesized with physical transducer model. Moderate enhancement results can already be obtained when applying this model to in vivo AR imaging data. Nevertheless, the perceptual quality is unsatisfactory due to domain shift. Further, domain transfer learning technique under generative adversarial network (GAN) framework is proposed to drive the enhanced image's manifold towards that of real OR image. In this way, perceptually convincing AR to OR enhancement result is obtained, which can also be supported by quantitative analysis. Peak Signal to Noise Ratio (PSNR) and Structural Similarity Index (SSIM) values are significantly increased from 14.74 dB to 19.01 dB and from 0.1974 to 0.2937, respectively, validating the improvement of reconstruction correctness and overall perceptual quality. The proposed algorithm has also been validated across different imaging depths with experiments conducted in both shallow and deep tissue. The above AR to OR domain transfer learning with GAN (AODTL-GAN) framework has enabled the enhancement target with limited amount of matched in vivo AR-OR imaging data.