MOTIVATION: Time-lapse microscopy is a powerful technique that relies on images of live cells cultured ex vivo that are captured at regular intervals of time to describe and quantify their behavior under certain experimental conditions. This imaging method has great potential in advancing the field of precision oncology by quantifying the response of cancer cells to various therapies and identifying the most efficacious treatment for a given patient. Digital image processing algorithms developed so far require high-resolution images involving very few cells originating from homogeneous cell line populations. We propose a novel framework that tracks cancer cells to capture their behavior and quantify cell viability to inform clinical decisions in a high-throughput manner. RESULTS: The brightfield microscopy images a large number of patient-derived cells in an ex vivo reconstruction of the tumor microenvironment treated with 31 drugs for up to 6 days. We developed a robust and user-friendly pipeline CancerCellTracker that detects cells in co-culture, tracks these cells across time and identifies cell death events using changes in cell attributes. We validated our computational pipeline by comparing the timing of cell death estimates by CancerCellTracker from brightfield images and a fluorescent channel featuring ethidium homodimer. We benchmarked our results using a state-of-the-art algorithm implemented in ImageJ and previously published in the literature. We highlighted CancerCellTracker's efficiency in estimating the percentage of live cells in the presence of bone marrow stromal cells. AVAILABILITY AND IMPLEMENTATION: https://github.com/compbiolabucf/CancerCellTracker. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

We introduce two variants of proper colorings with imposed partial ordering on the set of colors. One variant shows very close connections to some fundamental problems in graph theory, e.g., directed graph homomorphism and list colorings. We study the border between tractability and intractability for both variants.

Electronic educational games can be highly entertaining, but studies have shown that they do not always trigger learning. To enhance the effectiveness of educational games, we propose intelligent pedagogical agents that can provide individualized instruction integrated with the entertaining nature of the games. In this paper, we describe one such agent, that we have developed for Prime Climb, an educational game on number factorization. The Prime Climb agent relies on a probabilistic student model to generate tailored interventions aimed at helping students learn number factorization through the game. After describing the functioning of the agent and the underlying student model, we report the results of an empirical study that we performed to test the agent's effectiveness.