Cell instance segmentation (CIS) is crucial for identifying individual cell morphologies in histopathological images, providing valuable insights for biological and medical research. While unsupervised CIS (UCIS) models aim to reduce the heavy reliance on labor-intensive image annotations, they fail to accurately capture cell boundaries, causing missed detections and poor performance. Recognizing the absence of error-free instances as a key limitation, we present COIN (COnfidence score-guided INstance distillation), a novel annotation-free framework with three key steps: (1) Increasing the sensitivity for the presence of error-free instances via unsupervised semantic segmentation with optimal transport, leveraging its ability to discriminate spatially minor instances, (2) Instance-level confidence scoring to measure the consistency between model prediction and refined mask and identify highly confident instances, offering an alternative to ground truth annotations, and (3) Progressive expansion of confidence with recursive self-distillation. Extensive experiments across six datasets show COIN outperforming existing UCIS methods, even surpassing semi- and weakly-supervised approaches across all metrics on the MoNuSeg and TNBC datasets. The code is available at https://github.com/shjo-april/COIN.

Weakly-supervised semantic segmentation aims to reduce labeling costs by training semantic segmentation models using weak supervision, such as image-level class labels. However, most approaches struggle to produce accurate localization maps and suffer from false predictions in class-related backgrounds (i.e., biased objects), such as detecting a railroad with the train class. Recent methods that remove biased objects require additional supervision for manually identifying biased objects for each problematic class and collecting their datasets by reviewing predictions, limiting their applicability to the real-world dataset with multiple labels and complex relationships for biasing. Following the first observation that biased features can be separated and eliminated by matching biased objects with backgrounds in the same dataset, we propose a fully-automatic/model-agnostic biased removal framework called MARS (Model-Agnostic biased object Removal without additional Supervision), which utilizes semantically consistent features of an unsupervised technique to eliminate biased objects in pseudo labels. Surprisingly, we show that MARS achieves new state-of-the-art results on two popular benchmarks, PASCAL VOC 2012 (val: 77.7%, test: 77.2%) and MS COCO 2014 (val: 49.4%), by consistently improving the performance of various WSSS models by at least 30% without additional supervision. Code is available at https://github.com/shjo-april/MARS.

Weakly-supervised semantic segmentation (WSSS) is introduced to narrow the gap for semantic segmentation performance from pixel-level supervision to image-level supervision. Most advanced approaches are based on class activation maps (CAMs) to generate pseudo-labels to train the segmentation network. The main limitation of WSSS is that the process of generating pseudo-labels from CAMs that use an image classifier is mainly focused on the most discriminative parts of the objects. To address this issue, we propose Puzzle-CAM, a process that minimizes differences between the features from separate patches and the whole image. Our method consists of a puzzle module and two regularization terms to discover the most integrated region in an object. Puzzle-CAM can activate the overall region of an object using image-level supervision without requiring extra parameters. In experiments, Puzzle-CAM outperformed previous state-of-the-art methods using the same labels for supervision on the PASCAL VOC 2012 dataset. Code associated with our experiments is available at https://github.com/OFRIN/PuzzleCAM.