BACKGROUND: Glucocorticoid-remediable aldosteronism (GRA) is a form of heritable hypertension caused by a chimeric fusion resulting from unequal crossing over between 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2), which are two genes with similar sequences. Different crossover patterns of the CYP11B1 and CYP11B2 chimeric genes may be associated with a variety of clinical presentations. It is therefore necessary to develop an efficient approach for identifying the differences between the hybrid genes of a patient with GRA. RESULTS: We developed a long-read analysis pipeline named GRAde (GRA deciphering), which utilizes the nonidentical bases in the CYP11B1 and CYP11B2 genomic sequences to identify and visualize the chimeric form. We sequenced the polymerase chain reaction (PCR) products of the CYP11B1/CYP11B2 chimeric gene from 36 patients with GRA using the Nanopore MinION device and analyzed the sequences using GRAde. Crossover events were identified for 30 out of the 36 samples. The crossover sites appeared in the region exhibiting high sequence similarity between CYP11B1 and CYP11B2, and 53.3% of the cases were identified as having a gene conversion in intron 2. More importantly, there were six cases for whom the PCR products indicated a chimeric gene, but the GRAde results revealed no crossover pattern. The crossover regions were further verified by Sanger sequencing analysis. CONCLUSIONS: PCR-based target enrichment followed by long-read sequencing is an efficient and precise approach to dissecting complex genomic regions, such as those involved in GRA mutations, which could be directly applied to clinical diagnosis. The scripts of GRAde are available at https://github.com/hsu-binfo/GRAde .

The live video streaming services have been suffered from the limited backhaul capacity of the cellular core network and occasional congestions due to the cloud-based architecture. Mobile Edge Computing (MEC) brings the services from the centralized cloud to nearby network edge to improve the Quality of Experience (QoE) of cloud services, such as live video streaming services. Nevertheless, the resource at edge devices is still limited and should be allocated economically efficiently. In this paper, we propose Edge Combinatorial Clock Auction (ECCA) and Combinatorial Clock Auction in Stream (CCAS), two auction frameworks to improve the QoE of live video streaming services in the Edge-enabled cellular system. The edge system is the auctioneer who decides the backhaul capacity and caching space allocation and streamers are the bidders who request for the backhaul capacity and caching space to improve the video quality their audiences can watch. There are two key subproblems: the caching space value evaluations and allocations. We show that both problems can be solved by the proposed dynamic programming algorithms. The truth-telling property is guaranteed in both ECCA and CCAS. The simulation results show that the overall system utility can be significantly improved through the proposed system.

Fog computing is proposed to mitigate cloud micro services, such as IoT gateway or AR data cache, from the centralized cloud to nearby base station (BS) or devices. The mobile users' devices can access service from nearby devices directly through Device to Device (D2D) communications. However, the incentive of devices to provide micro service is a challenge since the devices would spend their storage, computation, and energy resource. In this paper, we propose Fog Micro Service Market Game (FoMG), a game-theoretic framework to promote Fog computing in Fog-enabled cellular system on mobile devices through free market mechanism. The mobile users may decide the micro services they are going to rent (renting strategy) and the price of the services (pricing strategy) freely. The optimal strategy and Nash equilibrium of FoMG are derived through proposed algorithms. The simulation results showed that the overall system utility can be significantly improved through the proposed system.