Immersive videos (IVs) provide 360° environments that create a strong sense of presence and spatial exploration. Unlike traditional videos, IVs distribute information across multiple directions, making comparison cognitively demanding and highly dependent on interaction techniques. With the growing adoption of IVs, effective comparison techniques have become an essential yet underexplored area of research. Inspired by the “sliding” concept in 2D media comparison, we integrate two established comparison strategies from the literature—toggle and side-by-side—to support IV comparison with greater flexibility. For an in-depth understanding of different strategies, we adapt and implement five IV comparison techniques across VR and 2D environments: SlideInVR, ToggleInVR, SlideIn2D, ToggleIn2D, and SideBySideIn2D. We then conduct a user study (N = 20) to examine how these techniques shape users’ perceptions, strategies, and workflows. Our findings provide empirical insights into the strengths and limitations of each technique, underscoring the need to switch between comparison approaches across scenarios. Notably, participants consistently rate SlideInVR and SlideIn2D as the most flexible and favorite methods for IV comparison.

MOTIVATION: The growing number of single-cell RNA-seq (scRNA-seq) studies highlights the potential benefits of integrating multiple datasets, such as augmenting sample sizes and enhancing analytical robustness. Inherent diversity and batch discrepancies within samples or across studies continue to pose significant challenges for computational analyses. Questions persist in practice, lacking definitive answers: Should we use a specific integration method or opt for simply merging the datasets during joint analysis? Among all the existing data integration methods, which one is more suitable in specific scenarios? RESULT: To fill the gap, we introduce SCIntRuler, a novel statistical metric for guiding the integration of multiple scRNA-seq datasets. SCIntRuler helps researchers make informed decisions regarding the necessity of data integration and the selection of an appropriate integration method. Our simulations and real data applications demonstrate that SCIntRuler streamlines decision-making processes and facilitates the analysis of diverse scRNA-seq datasets under varying contexts, thereby alleviating the complexities associated with the integration of heterogeneous scRNA-seq datasets. AVAILABILITY AND IMPLEMENTATION: The implementation of our method is available on CRAN as an open-source R package with a user-friendly manual available: https://cloud.r-project.org/web/packages/SCIntRuler/index.html.

MOTIVATION: Spatial transcriptomics has greatly contributed to our understanding of spatial and intra-sample heterogeneity, which could be crucial for deciphering the molecular basis of human diseases. Intra-tumor heterogeneity, e.g. may be associated with cancer treatment responses. However, the lack of computational tools for exploiting cross-regional information and the limited spatial resolution of current technologies present major obstacles to elucidating tissue heterogeneity. RESULTS: To address these challenges, we introduce RegionalST, an efficient computational method that enables users to quantify cell type mixture and interactions, identify sub-regions of interest, and perform cross-region cell type-specific differential analysis for the first time. Our simulations and real data applications demonstrate that RegionalST is an efficient tool for visualizing and analyzing diverse spatial transcriptomics data, thereby enabling accurate and flexible exploration of tissue heterogeneity. Overall, RegionalST provides a one-stop destination for researchers seeking to delve deeper into the intricacies of spatial transcriptomics data. AVAILABILITY AND IMPLEMENTATION: The implementation of our method is available as an open-source R/Bioconductor package with a user-friendly manual available at https://bioconductor.org/packages/release/bioc/html/RegionalST.html.

HJ-1B Infrared Scanner (IRS) has accumulated long-term data, but there are no comparative studies on land surface temperature (LST) inversion algorithms for IRS data. This study compared the radiative transfer equation (RTE) and two generalized single-channel algorithms (GSCwand GSCwT) for retrieving LST from IRS data. Firstly, the coefficients of the GSCwand the GSCwTalgorithms were simulated using MODTRAN 5.2 and the TIGR atmospheric profiles. ERA5 atmospheric profiles were used for the RTE algorithm. Second, land surface emissivity was calculated using the ASTER global emissivity dataset and vegetation/snow cover products based on the vegetation cover method. Finally, the LST retrievals were evaluated using ground measurements of twelve sites during the Heihe Watershed Allied Telemetry Experimental Research (HiWATER) experiment from June 2012 to June 2014. The results showed that the accuracy of the RTE with bias (RMSE) of 0.74 K (2.47 K) is superior to the accuracy of the GSCwT with bias (RMSE) of -1.18 K (2.50 K), followed by the GSCwwith bias (RMSE) of 1.60 K (2.77 K). The results can support the production of HJ-1B/IRS LST products.