Abstract Sumary: A number of multifunctional, ‘moonlighting’, proteins have been analyzed by different current programs to test wheter they identify both functions. PSI-BLAST and PRODOM perform best in predicting the alternative function. Contact: [email protected] Supplementary information: Full tables and additional information available at http://ibb.uab.es/moonlighting * The authors wish it to be known that, in their opinion, the first two authors should be regarded as joint First Authors. † To whom correspondence should be addressed.

Abstract Summary: ASAP is a web tool designed to search for specific dipeptides, tripeptides and tetrapeptides in a protein sequence database. The server allows for: (a) identification of frequent and infrequent peptides and the creation of peptide probability tables for a given database of sequences (GenerNet program), (b) determination of the compatibility of an amino-acid sequence to the given peptide probability tables (ClonErrNet program); and (c) comparison of different protein databases based on peptide composition (CompNet program). ASAP server can be useful in protein engineering and/or protein classification studies. Availability: http://guitar.rockefeller.edu/~marcius/ASAP.html Contact: [email protected] * To whom correspondence should be addressed.