Processing-using-DRAM (PuD) is a promising paradigm for alleviating the data movement bottleneck using a DRAM array's massive internal parallelism and bandwidth to execute very wide dataparallel operations.Performing a PuD operation involves activating multiple DRAM rows in quick succession or simultaneously, i.e., multiple-row activation.Multiple-row activation is fundamentally different from conventional memory access patterns that activate one DRAM row at a time.However, repeatedly activating even one DRAM row (e.g., RowHammer) can induce bitflips in unaccessed DRAM rows because modern DRAM is subject to read disturbance, a worsening safety, security, and reliability issue.Unfortunately, no prior work investigates the effects of multiple-row activation, as commonly used by PuD operations, on DRAM read disturbance.In this paper, we present the first characterization study of read disturbance effects of multiple-row activation-based PuD (which we call PuDHammer) using 316 real DDR4 DRAM chips from four major DRAM manufacturers.Our detailed characterization results covering various operational conditions and parameters (i.e., temperature, data patterns, access patterns, timing parameters, and spatial variation) show that 1) PuDHammer significantly exacerbates the read disturbance vulnerability, causing up to 158.58× reduction in the minimum hammer count required to induce the first bitflip (𝐻𝐶 𝑓 𝑖𝑟𝑠𝑡 ), compared to RowHammer, 2) PuDHammer is affected by various operational conditions and parameters, 3) combining RowHammer with PuDHammer is more effective than using