MOTIVATION: RNA-protein interactions play essential roles in every living organism, with RNA transcription, processing, and translation being just a few examples. Therefore, determining the set of RNAs that are bound by individual RNA-binding proteins, as well as the precise location of the interaction, is crucial for biological understanding. CLIP (UV-cross-linking and immunoprecipitation) is a method developed to study these interactions. Several variations of the CLIP protocol have been developed, e.g. iCLIP (individual-nucleotide resolution CLIP), which offers nucleotide-precise resolution of the cross-linking event. RESULTS: PARANOiD is a versatile software for fully automated analysis of iCLIP and iCLIP2 data. It contains all steps necessary for preprocessing, the determination of cross-link locations, and several additional steps, which can be used to detect specific characteristics, e.g. definite distances between cross-link events or identify binding motifs. Additionally, results are visualized as statistical plots for a quick overview and as standardized bioinformatics file formats, which can be used for further analysis steps. AVAILABILITY AND IMPLEMENTATION: PARANOiD is published under the MIT license and is available from https://github.com/patrick-barth/PARANOiD. The documentation is available at https://paranoid.readthedocs.io/en/latest/index.html.

MOTIVATION AND RESULTS: Crypt4GH-JS is a browser-ready implementation of the Crypt4GH file encryption standard written in JavaScript. While having minimal to no impact on data upload and download throughput this library enables on-the-fly encryption of arbitrary data in web applications, regardless of whether on the client or server side. As development moves more and more toward cloud-native applications, this library represents a significant step forward for flexible data security in the context of opaque cloud storage systems. AVAILABILITY AND IMPLEMENTATION: Crypt4GH-JS can be installed via Node Package Manager (https://www.npmjs.com/package/crypt4gh_js) or through its public GitHub Repository (https://github.com/fathelen/crypt4ghJS), where the source code is available. Crypt4GH-JS can be tested in the browser using our demonstration website, which can be found at: https://fathelen.github.io/crypt4ghJS/.