SUMMARY: Here, we present PhyloWGA, an open source R package for conducting phylogenetic analysis and investigation of whole genome data. AVAILABILITYAND IMPLEMENTATION: Available at Github (https://github.com/radamsRHA/PhyloWGA). SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

Summary: We describe ThetaMater, an open source R package comprising a suite of functions for efficient and scalable Bayesian estimation of the population size parameter θ from genomic data. Availability and implementation: ThetaMater is available at GitHub (https://github.com/radamsRHA/ThetaMater). Contact: [email protected]. Supplementary information: Supplementary data are available at Bioinformatics online.

Summary: We introduce GppFst, an open source R package that generates posterior predictive distributions of FST and dx under a neutral coalescent model to identify putative targets of selection from genomic data. Availability and Implementation: GppFst is available at ( https://github.com/radamsRHA/GppFst ). Contact: [email protected]. Supplementary information: Supplementary data are available at Bioinformatics online.

SUMMARY: Phylogenetics, likelihood, evolution and complexity (PLEX) is a flexible and fast Bayesian Markov chain Monte Carlo software program for large-scale analysis of nucleotide and amino acid data using complex evolutionary models in a phylogenetic framework. The program gains large speed improvements over standard approaches by implementing 'partial sampling of substitution histories', a data augmentation approach that can reduce data analysis times from months to minutes on large comparative datasets. A variety of nucleotide and amino acid substitution models are currently implemented, including non-reversible and site-heterogeneous mixture models. Due to efficient algorithms that scale well with data size and model complexity, PLEX can be used to make inferences from hundreds to thousands of taxa in only minutes on a desktop computer. It also performs probabilistic ancestral sequence reconstruction. Future versions will support detection of co-evolutionary interactions between sites, probabilistic tests of convergent evolution and rigorous testing of evolutionary hypotheses in a Bayesian framework. AVAILABILITY AND IMPLEMENTATION: PLEX v1.0 is licensed under GPL. Source code and documentation will be available for download at www.evolutionarygenomics.com/ProgramsData/PLEX. PLEX is implemented in C++ and supported on Linux, Mac OS X and other platforms supporting standard C++ compilers. Example data, control files, documentation and accessory Perl scripts are available from the website. CONTACT: [email protected]. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.