Although video affective content analysis has great potential in many applications, it has not been thoroughly studied due to limited datasets. In this paper, we construct a large-scale video affective dataset with danmu (VAD). It consists of 19,267 elaborately segmented video clips from user-generated videos. The VAD dataset is annotated by the crowdsourcing platform with discrete valence, arousal, and primary emotions, as well as the comparison of valence and arousal between two consecutive video clips. Unlike previous datasets, including only video clips, our proposed dataset also provides danmu, which is the real-time comment from users as they watch a video. Danmu provides extra information for video affective content analysis. As a preliminary assessment of the usability of our dataset, an analysis of inter-annotator consistency for each label is conducted using weighted Fleiss' Kappa, regular Fleiss' Kappa, intraclass correlation coefficient, and percent consensus. Besides, we also perform a statistical analysis of labels and danmu. Finally, video affective content analysis is conducted on our dataset and three typical methods (i.e., TFN, MulT, and MISA) are leveraged to provide benchmarks. We also demonstrate that danmu can significantly improve the performance of the video affective content analysis task on some labels. Our dataset is available for research purposes.

Although empathic interaction between counselor and client is fundamental to success in the psychotherapeutic process, there are currently few datasets to aid a computational approach to empathy understanding. In this paper, we construct a multimodal empathy dataset collected from face-to-face psychological counseling sessions. The dataset consists of 771 video clips. We also propose three labels (i.e., expression of experience, emotional reaction, and cognitive reaction) to describe the degree of empathy between counselors and their clients. Expression of experience describes whether the client has expressed experiences that can trigger empathy, and emotional and cognitive reactions indicate the counselor's empathic reactions. As an elementary assessment of the usability of the constructed multimodal empathy dataset, an interrater reliability analysis of annotators' subjective evaluations for video clips is conducted using the intraclass correlation coefficient and Fleiss' Kappa. Results prove that our data annotation is reliable. Furthermore, we conduct empathy prediction using three typical methods, including the tensor fusion network, the sentimental words aware fusion network, and a simple concatenation model. The experimental results show that empathy can be well predicted on our dataset. Our dataset is available for research purposes.

MOTIVATION: When identifying differentially expressed (DE) genes from high-throughput gene expression measurements, we would like to take both statistical significance (such as P-value) and biological relevance (such as fold change) into consideration. In gene set enrichment analysis (GSEA), a score that can combine fold change and P-value together is needed for better gene ranking. RESULTS: We defined a gene significance score π-value by combining expression fold change and statistical significance (P-value), and explored its statistical properties. When compared to various existing methods, π-value based approach is more robust in selecting DE genes, with the largest area under curve in its receiver operating characteristic curve. We applied π-value to GSEA and found it comparable to P-value and t-statistic based methods, with added protection against false discovery in certain situations. Finally, in a gene functional study of breast cancer profiles, we showed that using π-value helps elucidating otherwise overlooked important biological functions. AVAILABILITY: http://gccri.uthscsa.edu/Pi_Value_Supplementary.asp CONTACT: [email protected], [email protected] SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.

MOTIVATION: A network is said to be robust relative to a certain network characteristic if a small change in network structure does not significantly affect the characteristic. From the perspective of network stability, robustness is desirable; however, from the perspective of intervention to exert influence on network behavior, it is undesirable. For Boolean networks, there are two fundamental types of robustness. One type pertains to perturbing the state of the network and the other to perturbing the rule-based structure. RESULTS: This article explores the impact of function perturbations in Boolean networks from two aspects: (1) analysis: predict the impact on network state transitions and attractors via analytical approaches or identify a perturbation by observing its consequences; (2) synthesis: preserve or modify the network characteristics, especially attractors, by introducing a judicious change to the functions. The results are applied to achieve intervention that structurally alters the network to achieve a more favorable steady-state distribution and to identify the function perturbation that has led to altered observed behavior. The intervention procedure is applied to a WNT5A network to reduce the risk of metastasis in melanoma, and the identification procedure is applied to a Drosophila melanogaster segmentation polarity gene network to identify regulatory function perturbation.