Summary: CRISPR-Cas9 and shRNA high-throughput sequencing screens have abundant applications for basic and translational research. Methods and tools for the analysis of these screens must properly account for sequencing error, resolve ambiguous mappings among similar sequences in the barcode library in a statistically principled manner, and be computationally efficient. Herein we present bcSeq, an open source R package that implements a fast and parallelized algorithm for mapping high-throughput sequencing reads to a barcode library while tolerating sequencing error. The algorithm uses a Trie data structure for speed and resolves ambiguous mappings by using a statistical sequencing error model based on Phred scores for each read. Availability and implementation: The package source code and an accompanying tutorial are available at http://bioconductor.org/packages/bcSeq/. Supplementary information: Supplementary data are available at Bioinformatics online.

This paper describes a human-in-the-loop evaluation of a power plant Human-Machine Interface (HMI), that was designed using a User-Centered Design approach. General Electric (GE) developed ActivePoint* HMI (AP HMI) to increase plant operator efficiency and awareness. UCD was applied as a design process along with existing industry design guidelines and other design approaches. The goal of the evaluation study was to provide GE Power quantitative data on proposed benefits from the new HMI based on systematic design approaches compared to the legacy HMI product, developed by engineers with an ad hoc process. Compared to this Legacy HMI, the new AP HMI provides enhanced information architecture, simplified graphics and navigation, as well as improved alarm management allowing operators and plant maintenance personnel to focus on what is important. Nine operations staff from a power plant in France performed simulated tasks using both Legacy HMI as a baseline and AP HMI. We measured their performance and satisfaction on the use of HMI to quantify the enhanced effectiveness of AP HMI. AP HMI resulted in 33% higher success rate, 79% lower navigation time, 42% lower total time on task compared to Legacy HMI. Also, the user satisfaction for AP HMI was rated significantly higher than Legacy HMI.

This study suggests a way to utilize the map of technology as a guide to find new technology component. Recent studies of mapping knowledge mainly focused on analyzing the map as a result of technological innovation rather than utilizing the map for exploring the world of technological innovation. The preliminary result of a case study suggests that a firm can find possible technology components that can be combined with own technology component. The map of technology comprises the nodes of International Patent Classification (IPC) main groups and the links presenting the co-assign relationship between the IPC main groups.