The design, development, sea trials and exploitation of the SESAMO (SEa Surface Autonomous MOdular unit) platform, an autonomous surface vessel for the study and characterization of the air-sea interface, are presented. The SESAMO prototype robot, developed by the robotics group of CNR-ISSIA, Genova branch, in strict cooperation with the scientific end-users in the framework of a project of the Italian National Program of Research in Antarctica (PNRA), was able to sample the sea surface microlayer and immediate sub-surface. After satisfactory basic at field trials of the navigation, guidance and control (NGC) and sampling systems, the robotised catamaran was exploited by the scientific end-users for water sample collection in the area of Terra Nova Bay, Ross Sea, Antarctica, in the framework of the PNRA project Chemical contamination.