Nanotechnology is one of the most promising technologies for future development. This paper proposes virtual reality (VR) as a tool to simulate nano particle manipulation using optical tweezers towards achieving nano-assembly and to handle effectively issues such as difficulty in viewing, perceiving and controlling the nano-scale objects. The simulation modeled using virtual reality displays all the forces acting on nanoparticle during the manipulation. The simulation is developed for particles that belong to the Rayleigh region and represents interactions of OT (a laser beam) with the nanoparticle. The laser beam aimed on to the nanoparticle traps the particle by applying optical forces. The trapped particle is then moved by moving the laser beam. The proposed VR based simulation tool with it capabilities can be easily extended and used for creating and open system framework by connecting it to a real OT setup to control nanoparticles manipulation. In addition, a feedback system can be build to increase of precision of movement.