Abstract Summary Spike-in controls are synthetic nucleic-acid sequences that are added to a user’s sample and constitute internal standards for subsequent steps in the next generation sequencing workflow. The Anaquin software toolkit can be used to analyze the performance of spike-in controls at multiple steps during RNA sequencing or genome sequencing analysis, providing useful diagnostic statistics, data visualization and sample normalization. Availability and Implementation The software is implemented in C ++/R and is freely available under BSD license. The source code is available from github.com/student-t/Anaquin, binaries and user manual from www.sequin.xyz/software and R package from bioconductor.org/packages/Anaquin Supplementary information Supplementary data are available at Bioinformatics online.

While there has been a nominal effort to develop automatic target recognition (ATR) technologies for military systems for the last couple of decades, there have been relatively few significant breakthroughs. At the same time, the evolution of some of our missions has made it more desirable to achieve solutions from this technology to meet some of the escalating operational challenges. This paper is intended to identify and characterize some areas where ATR algorithms might materially improve our operational capability